CAJ | 학술논문

【目的】探讨ω-3鱼油脂肪乳剂对内毒素脂多糖（LPS）致兔急性肺损伤（ALI）急性肺损伤兔肺组织中血栓素B2（TXB2）、6酮前列腺素F1α（6-keto-PGF1α）表达的影响。【方法】清洁级新西兰兔24只，随机分为对照组（A组）、LPS致伤组（B组）、ω-3鱼油脂肪乳剂预处理＋LPS致伤组（C组），每组8只，记录0h、0．5h、1h、2h、4h时间点氧和指数（PaO2/FiO2）的改变，4h点处死动物，取肺组织测定TXB2和6-酮-前列腺素F1α（6-keto-PGF1α）含量、观察病理变化，并做病理评分。【结果】注射LPS0．5h、1h后B、C两组0．5h、1h兔肺组织TXB2、6-keto-PGF1α均较A组明显升高（P＜0．05），B、C两组比较有统计学意义（P＜0．05），注射LPS2h、4h后三组比较无统计学意义（P＞0．05）；B组TXB2/6-keto-PGF1α（T/P）明显高于A、C两组（P＜0．05），A组与C组T/P均有增高趋势（0．75VS0．73），但两组比较无显著差异（P＞0．05）；A组、C组肺损伤病理组织评分均低于B组（P＜0．05）。【结论】ω-3鱼油脂肪乳可明显减少LPS致ALI的肺组织TXB2含量，对6-keto-PGF1α最终合成无明显抑制，从而显著下调T/P，对内毒素致兔ALI具有一定保护作用。
【목적】탐토ω-3어유지방유제대내독소지다당（LPS）치토급성폐손상（ALI）급성폐손상토폐조직중혈전소B2（TXB2）、6동전렬선소F1α（6-keto-PGF1α）표체적영향。【방법】청길급신서란토24지，수궤분위대조조（A조）、LPS치상조（B조）、ω-3어유지방유제예처리＋LPS치상조（C조），매조8지，기록0h、0．5h、1h、2h、4h시간점양화지수（PaO2/FiO2）적개변，4h점처사동물，취폐조직측정TXB2화6-동-전렬선소F1α（6-keto-PGF1α）함량、관찰병리변화，병주병리평분。【결과】주사LPS0．5h、1h후B、C량조0．5h、1h토폐조직TXB2、6-keto-PGF1α균교A조명현승고（P＜0．05），B、C량조비교유통계학의의（P＜0．05），주사LPS2h、4h후삼조비교무통계학의의（P＞0．05）；B조TXB2/6-keto-PGF1α（T/P）명현고우A、C량조（P＜0．05），A조여C조T/P균유증고추세（0．75VS0．73），단량조비교무현저차이（P＞0．05）；A조、C조폐손상병리조직평분균저우B조（P＜0．05）。【결론】ω-3어유지방유가명현감소LPS치ALI적폐조직TXB2함량，대6-keto-PGF1α최종합성무명현억제，종이현저하조T/P，대내독소치토ALI구유일정보호작용。
[Objective]To explore the influence of ω-3 fish oil fat emulsion on the expression of TXB2 and 6-keto-PGF1α in lung tissues of acute lung injury (ALI) in rabbits induced by lipopolysaccharide(LPS) .[Meth-ods] A total of 24 clean-grade New Zealand rabbits were randomly divided into control group(group A) ,LPS injury group(group B) and ω-3 fish oil fat emulsion pretreatment and LPS injury group (group C) with 8 cases in each group .The change of oxygenation index(PaO2/FiO2 ) at 0h ,0 .5h ,1h ,2h and 4h time point was re-corded .The animals were killed at 4h .The lung tissues were collected to detect the levels of TXB 2 and 6-keto-PGF1α .The pathological changes were observed and the pathological scores were obtained .[Results] Com-pared with group A ,TXB2 and 6-keto-PGF1α in lung tissues of group B and group C after injection with LPS for 0 .5h and 1h were obviously increased( P <0 .05) ,and there were significant differences between group B and group C( P <0 .05) .There was no significant difference among three groups after injection with LPS for 2h and 4h( P >0 .05) .TXB2/6-keto-PGF1α(T/P) in group B was obviously higher than that in group A and group C( P <0 .05) .The T/P in group A and group C had an increasing tendency(0 .75 vs .0 .73) ,but there was no significant difference between two groups ( P >0 .05) .The histopathological scores of lung injury in group A and group C were lower than those in group B( P<0 .05) .[Conclusion] Theω-3 fish oil fat emulsion can obviously reduce the level of TXB2 in lung tissues of LPS-induced ALI ,but has no obvious inhibition on fi-nal synthesis of 6-keto-PGF1α ,thereby significantly reduce T/P ,and has protective effect on endotoxin-in-duced ALI in rabbits .