CAJ | 학술논문

目的：探讨环氧合酶-2（COX-2）基因-1195 G>A多态性位点与缺血性脑卒中（IS）患者阿司匹林抵抗（AR）的相关性。方法：纳入中国北方汉族IS患者210例，根据血小板聚集率分为阿司匹林敏感（AS）组147例，非AS组63例。采用扩增阻碍突变系统（ARMS）结合TaqMan探针的实时荧光定量PCR方法检测-1195 G>A多态性。结果：-1195G>A位点基因型、显性模型分布频率在AS组及非AS组中差异均无统计学意义（＞0.05）；非AS组中-1195G>A位点（GA+AA）基因型频率及A等位基因频率高于AS组，差异均有统计学意义（x2=4.96，=0.03；x2=5.50，=0.02）；多因素分析提示，与GG基因型相比，COX-2的-1195G>A位点（GA+AA）基因型发生AR可能性更大（=2.41，
목적：탐토배양합매-2（COX-2）기인-1195 G>A다태성위점여결혈성뇌졸중（IS）환자아사필림저항（AR）적상관성。방법：납입중국북방한족IS환자210례，근거혈소판취집솔분위아사필림민감（AS）조147례，비AS조63례。채용확증조애돌변계통（ARMS）결합TaqMan탐침적실시형광정량PCR방법검측-1195 G>A다태성。결과：-1195G>A위점기인형、현성모형분포빈솔재AS조급비AS조중차이균무통계학의의（＞0.05）；비AS조중-1195G>A위점（GA+AA）기인형빈솔급A등위기인빈솔고우AS조，차이균유통계학의의（x2=4.96，=0.03；x2=5.50，=0.02）；다인소분석제시，여GG기인형상비，COX-2적-1195G>A위점（GA+AA）기인형발생AR가능성경대（=2.41，
ObjectiveTo study the correlation between single nucleotide polymorphisms (SNPs) of-1195G>A of cyclooxygenase-2 (COX-2) gene and Aspirin resistance (AR) in patients with ischemic stroke (IS). Methods:According to the rate of platelet aggregation, 210 northern Chinese Han patients with IS were divided into Aspirin sensitivity (AS) group (147 cases) and non-Asgroup [17 AR cases, and 46 Aspirin semi-resistance (ASR) cases] (n=63). The SNPs were detected by Amplification mutation system (ARMS) combined with TaqMan probe in a real-time PCR. Results:There were no significant differences in the genotype, allele, additive or recessive model frequencies of-1195G>A polymorphisms between the 2 groups. The significant difference between (GA+AA) and GG genotype frequencies of-1195G>A polymorphisms were observed in groups of AS and non-AS ( =0.03). For A allele of-1195G>A, there was also a significant difference between the two groups ( =0.02). On multivariate analysis controlling for conventional vascular risk factors, the-1195 GA and AA genotypes were identified inde-pendently to be related with (ASR+AR) ( =2.41,:1.07~5.42, =0.03). Conclusion:-1195G>A poly-morphism of COX-2 gene was associated with AR or ASR in patients with IS, which may be a genetic predisposi-tion to phenotypes of AR or ASR.