神经损伤与功能重建
神經損傷與功能重建
신경손상여공능중건
NEURAL INJURY AND FUNCTIONAL RECONSTRUCTION
2014年
2期
100-104
,共5页
张小乔%李鹂%刘伟%陈敏
張小喬%李鸝%劉偉%陳敏
장소교%리리%류위%진민
重复经颅磁刺激%血管性痴呆%学习记忆功能%海马%胆碱能系统
重複經顱磁刺激%血管性癡呆%學習記憶功能%海馬%膽堿能繫統
중복경로자자격%혈관성치태%학습기억공능%해마%담감능계통
rrepetitive transcranial magnetic stimulation%vascular dementia%function of learning and meory%hippocampus%cholinergic system
目的：观察重复经颅磁刺激（rTMS）对血管性痴呆（VaD）大鼠学习记忆功能及海马胆碱能系统的影响，并探讨其可能的作用机制。方法：雄性SD大鼠54只，随机分为对照组、模型组、rTMS组，每组18只。采用两血管阻断法制作VaD模型，对照组仅分离暴露双侧颈总动脉而不结扎。rTMS组于制模成功后给予rTMS治疗，模型组模拟rTMS固定大鼠头部放置线圈但不给予脉冲磁刺激。各组在造模第30天应用Morris水迷宫试验检测学习记忆能力，测定海马内乙酰胆碱酯酶（AChE）及胆碱乙酰转移酶（ChAT）的活性，行海马CA1区胆碱酯酶阳性纤维染色及密度测定，应用免疫组化技术检测海马CA1区脑源性神经营养因子（BDNF）的表达。结果：与模型组相比，rTMS组水迷宫逃避潜伏期明显缩短（＜0.05），相同时间内在原平台象限跨越相应平台次数明显增多（＜0.05）；与对照组相比，模型组及rTMS组AChE及ChAT的活性均明显降低（＜0.05）；与模型组相比，rTMS组AChE及ChAT的活性均明显增加（＜0.05）；rTMS组海马CA1区乙酰胆碱酯酶阳性纤维的密度及BDNF的表达均较模型组明显增强（＜0.05）。结论：rTMS能改善VaD大鼠学习记忆功能，机制可能与rTMS治疗能促进海马CA1区BDNF的表达、恢复海马胆碱能系统活性有关。
目的：觀察重複經顱磁刺激（rTMS）對血管性癡呆（VaD）大鼠學習記憶功能及海馬膽堿能繫統的影響，併探討其可能的作用機製。方法：雄性SD大鼠54隻，隨機分為對照組、模型組、rTMS組，每組18隻。採用兩血管阻斷法製作VaD模型，對照組僅分離暴露雙側頸總動脈而不結扎。rTMS組于製模成功後給予rTMS治療，模型組模擬rTMS固定大鼠頭部放置線圈但不給予脈遲磁刺激。各組在造模第30天應用Morris水迷宮試驗檢測學習記憶能力，測定海馬內乙酰膽堿酯酶（AChE）及膽堿乙酰轉移酶（ChAT）的活性，行海馬CA1區膽堿酯酶暘性纖維染色及密度測定，應用免疫組化技術檢測海馬CA1區腦源性神經營養因子（BDNF）的錶達。結果：與模型組相比，rTMS組水迷宮逃避潛伏期明顯縮短（＜0.05），相同時間內在原平檯象限跨越相應平檯次數明顯增多（＜0.05）；與對照組相比，模型組及rTMS組AChE及ChAT的活性均明顯降低（＜0.05）；與模型組相比，rTMS組AChE及ChAT的活性均明顯增加（＜0.05）；rTMS組海馬CA1區乙酰膽堿酯酶暘性纖維的密度及BDNF的錶達均較模型組明顯增彊（＜0.05）。結論：rTMS能改善VaD大鼠學習記憶功能，機製可能與rTMS治療能促進海馬CA1區BDNF的錶達、恢複海馬膽堿能繫統活性有關。
목적：관찰중복경로자자격（rTMS）대혈관성치태（VaD）대서학습기억공능급해마담감능계통적영향，병탐토기가능적작용궤제。방법：웅성SD대서54지，수궤분위대조조、모형조、rTMS조，매조18지。채용량혈관조단법제작VaD모형，대조조부분리폭로쌍측경총동맥이불결찰。rTMS조우제모성공후급여rTMS치료，모형조모의rTMS고정대서두부방치선권단불급여맥충자자격。각조재조모제30천응용Morris수미궁시험검측학습기억능력，측정해마내을선담감지매（AChE）급담감을선전이매（ChAT）적활성，행해마CA1구담감지매양성섬유염색급밀도측정，응용면역조화기술검측해마CA1구뇌원성신경영양인자（BDNF）적표체。결과：여모형조상비，rTMS조수미궁도피잠복기명현축단（＜0.05），상동시간내재원평태상한과월상응평태차수명현증다（＜0.05）；여대조조상비，모형조급rTMS조AChE급ChAT적활성균명현강저（＜0.05）；여모형조상비，rTMS조AChE급ChAT적활성균명현증가（＜0.05）；rTMS조해마CA1구을선담감지매양성섬유적밀도급BDNF적표체균교모형조명현증강（＜0.05）。결론：rTMS능개선VaD대서학습기억공능，궤제가능여rTMS치료능촉진해마CA1구BDNF적표체、회복해마담감능계통활성유관。
ObjectiveTo investigate the effects of repetitive transcranial magnetic stimulation (rTMS) on the abilities of learning and memory and the cholinergic system of hippocampus formation in rats with vascular dementia (VaD) . Methods: Fifty-four male SD rats were randomly divided into control, VaD model and rTMS groups with 18 rats in each group. The VaD rats were established with two vessel occlusion method whereas bilat-eral carotid arteries were only exposed without ligation in the control group. The animals in the rTMS group were given rTMS treatment. The rats in the model group were treated by simulated rTMS and fixed head coil without impulse magnetic stimulation. Morris water maze (MWM) test was performed to evaluate learning and memory function of rats on the day 30 after two vessel occlusion. The activity of AchE and ChAT, the density of AChE-positive staining fiber and the expression of brain-derived neurotrophic factor (BDNF) in hippocampus were investigated. Results:The MWM escape latency in the rTMS group was shorter than that in the model group ( <0.05). The number of crossing the corresponding platform during the same time period in platform quad-rant in the rTMS group was significant more than that in the model group ( <0.05). The activity of AchE and ChAT was decreased significantly in the model group compared with those in the control group, whereas it was improved significantly in the rTMS group ( <0.05). The density of AChE-positive staining fiber and the expres-sion of BDNF in the rTMS group were higher than those in the model group ( <0.05). Conclusion:rTMS can ameliorate learning and memory deficit of rats with VaD, perhaps by way of increased expression of BDNF in hippocampus CA1 area and repair of hippocampal cholinergic system activity.
