CAJ | 학술논문

目的：采用激光共聚焦显微镜（LSCM）技术和免疫组化染色检测KCNQ1在小鼠耳蜗血管纹中的表达分布特征。方法：以KCNQ1-/-突变纯合子基因型小鼠和CBA/CaJ小鼠为实验对象，采用免疫组化染色、LSCM结合免疫学标记方法对比观察血管纹KCNQ1蛋白表达情况。结果：免疫组化染色显示CBA/CaJ小鼠血管纹边缘细胞顶膜KCNQ1蛋白高表达，呈棕褐色；LSCM观察显示免疫单标KCNQ1绿色荧光蛋白分布于CBA/CaJ小鼠血管纹边缘细胞细胞核，KCNQ1-/-小鼠边缘细胞均未见阳性反应。结论：LSCM技术能准确显示KCNQ1在小鼠耳蜗血管纹中表达及细胞定位。
목적：채용격광공취초현미경（LSCM）기술화면역조화염색검측KCNQ1재소서이와혈관문중적표체분포특정。방법：이KCNQ1-/-돌변순합자기인형소서화CBA/CaJ소서위실험대상，채용면역조화염색、LSCM결합면역학표기방법대비관찰혈관문KCNQ1단백표체정황。결과：면역조화염색현시CBA/CaJ소서혈관문변연세포정막KCNQ1단백고표체，정종갈색；LSCM관찰현시면역단표KCNQ1록색형광단백분포우CBA/CaJ소서혈관문변연세포세포핵，KCNQ1-/-소서변연세포균미견양성반응。결론：LSCM기술능준학현시KCNQ1재소서이와혈관문중표체급세포정위。
ObjectiveTo investigate the expression and distribution of KCNQ1 in stria vascularis of murine cochlea with laser scanning confocal microscopy (LSCM) and immunohistochemistry staining technique. Methods: With LSCM and immunohistochemistry staining, the location of KCNQ1 in cochlea of the CBA/CaJ and KCNQ1 -/ -mice were observed. Results: Immunohistochemical staining showed that the expression of KCNQ1 protein was found at the apical membrane wall of marginal cells in stria vascularis of CBA/CaJ mice. There was no KCNQ1 protein expression in marginal cells of KCNQ1 -/-mice. The fluorescence of KCNQl protein was mainly expressed in the cell membrane of marginal cells of CBA/CaJ mice with LSCM. Conclusion:The expression and distribution of KCNQ1 protein in marginal cells of stria vascularis could be observed with LSCM.