CAJ | 학술논문

目的探讨γ-IFN对兔盆腔常规分割照射后直肠放射性损伤及纤维化的影响.方法建立兔放射性直肠损伤的动物模型,52只新西兰兔分为常规照射组、γ-IFN组及正常对照组,除正常对照组外其余兔盆腔均接受6 MV X线照射.γ-IFN组从照射后第5天给予γ-IFN 250 000 U/kg,1次,周.常规照射组给予5 ml/kg的生理盐水.照射后4、8、12和16周处死兔,取其照射范围内的直肠.用HE染色、原位杂交染色及免疫组织化学方法分析γ-IFN对于放射性直肠损伤及纤维化影响.结果照射后4、8和12周γ-IFN组的放射性直肠损伤与常规照射组比较,差异均有统计学意义(P<0.05),16周差异无统计学意义(P>0.05).TGF-βl mRNA原位杂交γ-IFN组直肠黏膜的TGF-β1 mRNA表达在照射后4、8、12和16周的放射性直肠损伤与常规照射组和正常对照组比较差异均有统计学意义(P<0.05).Ⅲ型胶原免疫组织化学结果显示γ-IFN组与常规照射组和正常对照组比较差异有统计学意义(P<0.05).结论 γ-IFN可抑制照射后直肠黏膜TGF-β1 mRNA的升高,降低Ⅲ型胶原的表达,减轻放射性直肠损伤及纤维化的形成.
목적 탐토γ-IFN대토분강상규분할조사후직장방사성손상급섬유화적영향.방법 건립토방사성직장손상적동물모형,52지신서란토분위상규조사조、γ-IFN조급정상대조조,제정상대조조외기여토분강균접수6 MV X선조사.γ-IFN조종조사후제5천급여γ-IFN 250 000 U/kg,1차,주.상규조사조급여5 ml/kg적생리염수.조사후4、8、12화16주처사토,취기조사범위내적직장.용HE염색、원위잡교염색급면역조직화학방법분석γ-IFN대우방사성직장손상급섬유화영향.결과 조사후4、8화12주γ-IFN조적방사성직장손상여상규조사조비교,차이균유통계학의의(P<0.05),16주차이무통계학의의(P>0.05).TGF-βl mRNA원위잡교γ-IFN조직장점막적TGF-β1 mRNA표체재조사후4、8、12화16주적방사성직장손상여상규조사조화정상대조조비교차이균유통계학의의(P<0.05).Ⅲ형효원면역조직화학결과현시γ-IFN조여상규조사조화정상대조조비교차이유통계학의의(P<0.05).결론 γ-IFN가억제조사후직장점막TGF-β1 mRNA적승고,강저Ⅲ형효원적표체,감경방사성직장손상급섬유화적형성.
Objective To investigate the effect of γ-IFN on the radiation-induced injury and fibrosis of the rabbit rectum after the fractionated irradiation to pelvic cavity. Methods The animal model of radiation rectum injury was established. 52 New Zealand rabbits were divided into 3 groups as the normal control group, the conventional radiation group, and γ-IFN group. Except the normal control group, the pelvic cavity of the two other groups were irradiated with 6 MV X-rays. At 5 d post-irradiatio the γ-IFN group was administered with 250 000 U/kg of γ-IFN once a week, while the conventional radiation group with 5 ml of saline solution. The rabbits were executed after 4,8,12 and 16 weeks, and the recta in the irradiated field were taken out. HE dyeing, in-situ hybridization and immuno-histochemical methods were used to analyze the radiation-induced rectrum injury and fibrosis. Results At 4,8, and 12 weeks post-irradiation, the radiation rectum injury and fibrosis were significantly different between the conventional radiation group and γ-IFN group(P < 0.05), but not at 16 weeks (P > 0.05) . At 4, 8, 12 and 16 weeks post-irradiation, the expression of TGF-β_1 mRNA in-situ hybridization in the γ-IFN group were significantly different compared to the conventional radiation group and the normal control group (P <0.05) . The immuno-histochemical results of Col Ⅲ showed the significant difference among the y-IFN group and both the normal control group and conventional radiation group ( P < 0.05). Conclusions γ-IFN could reduce the radiation rectum injury, suppress the expression of TGF-β_1 mRNA in the rectum mucosa, inhibit the expression of Col Ⅲ,and delay the formation of radiation-induced fibrosis.