CAJ | 학술논문

目的：建立一种新方法来观察细胞之间的相互粘附作用。方法利用BioFlux数控剪切流条件下活细胞功能分析自动化系统，首先将C57骨髓间充质干细胞（C57-BMSC）、CD45+CD31+BMSC和CD45-CD31-BMSC三种细胞在微流体通道上包被，用Calcein-AM对DC细胞进行染色，将染色的细胞在特定剪切力下（0.5dynes/cm2）引入小室内，通过荧光倒置显微镜和BioFlux软件系统进行图像捕捉和数据分析。结果在荧光倒置显微镜下观察和BioFlux软件统计分析，结果显示CD45+CD31+BMSC与未分选的C57-BMSC粘附的DC数量上无差异，CD45-CD31-BMSC粘附的DC细胞明显减少。结论利用BioFlux数控剪切流条件下活细胞功能分析自动化系统可以直观观察细胞之间的粘附作用。
목적：건립일충신방법래관찰세포지간적상호점부작용。방법이용BioFlux수공전절류조건하활세포공능분석자동화계통，수선장C57골수간충질간세포（C57-BMSC）、CD45+CD31+BMSC화CD45-CD31-BMSC삼충세포재미류체통도상포피，용Calcein-AM대DC세포진행염색，장염색적세포재특정전절력하（0.5dynes/cm2）인입소실내，통과형광도치현미경화BioFlux연건계통진행도상포착화수거분석。결과재형광도치현미경하관찰화BioFlux연건통계분석，결과현시CD45+CD31+BMSC여미분선적C57-BMSC점부적DC수량상무차이，CD45-CD31-BMSC점부적DC세포명현감소。결론이용BioFlux수공전절류조건하활세포공능분석자동화계통가이직관관찰세포지간적점부작용。
Objective To establish a new method to observe the interaction between the marrow mesenchymal stem cells and DC cells. Methods BioFlux system is for live-cell assays under shear-flow .we first cultured C57 bone marrow mesenchymal stem cells (C57-BMSC), CD45+ CD31+ BMSC and CD45-CD31-BMSC three kinds of cells in the microfluidic channels. DC cells were stained by Calcein-AM and added into the chamber under a shear force (0.5dynes/cm2). Pictures were capured by fluorescence inverted. Data were analysised by BioFlux software. Results The number of DCs adhered with CD45+ CD31+ BMSC was no difference with unsorted C57-BMSC . The number of DCs adhered with CD45-CD31-BMSC significantly decreased than unsorted C57-BMSC. Conclusion Adhesion between cells can be visually observed by BioFlux system for live-cell assays under shear-flow.