浙江临床医学
浙江臨床醫學
절강림상의학
ZHEJIANG CLINICAL MEDICAL JOURNAL
2014年
3期
337-338,339
,共3页
肺炎克雷伯菌%KPC酶%Hodge试验%PCR
肺炎剋雷伯菌%KPC酶%Hodge試驗%PCR
폐염극뢰백균%KPC매%Hodge시험%PCR
Klebsiella pneumoniae%KPC carbapenemase%Hodge testing%PCR
目的：了解徐州地区三级医院肺炎克雷伯菌产KPC酶流行状况。方法收集徐州地区三级医院临床分离非重复肺炎克雷伯菌130株，予VITEK-32型全自动微生物分析系统鉴定，筛选出对亚胺培南耐药或中介菌株，对初筛阳性菌予改良Hodge试验进行表型确认，PCR方法检测KPC基因。结果初筛41株肺炎克雷伯菌可疑产碳青霉烯酶，经改良Hodge试验测定35株KPC酶阳性，检出率为26.9%（35/130），PCR方法检测有33株KPC酶阳性，检出率为25.4%（33/130）。结论徐州地区三级医院产KPC酶肺炎克雷伯菌发生率相对较高，需引起临床关注。
目的：瞭解徐州地區三級醫院肺炎剋雷伯菌產KPC酶流行狀況。方法收集徐州地區三級醫院臨床分離非重複肺炎剋雷伯菌130株，予VITEK-32型全自動微生物分析繫統鑒定，篩選齣對亞胺培南耐藥或中介菌株，對初篩暘性菌予改良Hodge試驗進行錶型確認，PCR方法檢測KPC基因。結果初篩41株肺炎剋雷伯菌可疑產碳青黴烯酶，經改良Hodge試驗測定35株KPC酶暘性，檢齣率為26.9%（35/130），PCR方法檢測有33株KPC酶暘性，檢齣率為25.4%（33/130）。結論徐州地區三級醫院產KPC酶肺炎剋雷伯菌髮生率相對較高，需引起臨床關註。
목적：료해서주지구삼급의원폐염극뢰백균산KPC매류행상황。방법수집서주지구삼급의원림상분리비중복폐염극뢰백균130주，여VITEK-32형전자동미생물분석계통감정，사선출대아알배남내약혹중개균주，대초사양성균여개량Hodge시험진행표형학인，PCR방법검측KPC기인。결과초사41주폐염극뢰백균가의산탄청매희매，경개량Hodge시험측정35주KPC매양성，검출솔위26.9%（35/130），PCR방법검측유33주KPC매양성，검출솔위25.4%（33/130）。결론서주지구삼급의원산KPC매폐염극뢰백균발생솔상대교고，수인기림상관주。
Objective To understand the prevalence of KPC carbapenemase in Klebsiella pneumoniae in Xuzhou region. Methods A total of 130 nonrepeated isolates of Klebsiella pneumoniae from tertiary hospitals in Xuzhou region were collected, imipenem-resistant were screened by VITEK-32,then use Hodge testing and PCR methods to detect KPC carbapenemase . Rwsults Among 130 isolates strains 41 isolates were deteced to imipenem-resistant,35 strains were detected to produce KPC carbapenemase by Hodge testing (26.9%),33strains were detected to produce KPC carbapenemase by PCR methods (25.4%). Conclusion The incidence of producing KPC carbapenemase of K. pneumoniae strains in Xuzhou area is relatively high and the control should be strengthened.
