CAJ | 학술논문

目的：比较非接触式共培养条件下两种同源间充质干细胞对退变髓核细胞生物学功能的影响。方法：取同一腰椎间盘突出症患者骨髓间充质干细胞（BMSCs）、脂肪间充质干细胞（ADSCs）和退变髓核细胞（NPCs），两种间充质干细胞分别与髓核细胞在Tanswell 6孔板中进行非接触式共培养，与ADSCs共培养的NPCs为A组，与BMSCs共培养的NPCs为B组，以单独培养的NPCs为对照组。共培养7d后，提取3组髓核细胞总RNA，进行反转录后，利用Real-Time PCR检测其Ⅱ型胶原、蛋白多糖和SOX-9基因的相对表达量。结果：非接触式共培养7d后，Ⅱ型胶原、蛋白多糖和SOX-9基因相对表达量对照组分别是1.03±0.28、1.21±0.40和0.94±0.34，A组分别是3.49±0.55、3.88±2.11和2.41±0.91，B组分别是7.60±1.89、6.26±2.96和4.55±1.88。与对照组相比，A、B组髓核细胞Ⅱ型胶原、蛋白多糖和SOX-9基因相对表达显著增加（P<0.05）；A、B两组间相比也有显著性差异（P<0.05）。结论：非接触式共培养条件下骨髓间充质干细胞和脂肪间充质干细胞对退变髓核细胞均有一定的激活效应；骨髓间充质干细胞对退变髓核细胞的激活效应更强，可能更加适合于椎间盘退行性疾病的生物学治疗。
목적：비교비접촉식공배양조건하량충동원간충질간세포대퇴변수핵세포생물학공능적영향。방법：취동일요추간반돌출증환자골수간충질간세포（BMSCs）、지방간충질간세포（ADSCs）화퇴변수핵세포（NPCs），량충간충질간세포분별여수핵세포재Tanswell 6공판중진행비접촉식공배양，여ADSCs공배양적NPCs위A조，여BMSCs공배양적NPCs위B조，이단독배양적NPCs위대조조。공배양7d후，제취3조수핵세포총RNA，진행반전록후，이용Real-Time PCR검측기Ⅱ형효원、단백다당화SOX-9기인적상대표체량。결과：비접촉식공배양7d후，Ⅱ형효원、단백다당화SOX-9기인상대표체량대조조분별시1.03±0.28、1.21±0.40화0.94±0.34，A조분별시3.49±0.55、3.88±2.11화2.41±0.91，B조분별시7.60±1.89、6.26±2.96화4.55±1.88。여대조조상비，A、B조수핵세포Ⅱ형효원、단백다당화SOX-9기인상대표체현저증가（P<0.05）；A、B량조간상비야유현저성차이（P<0.05）。결론：비접촉식공배양조건하골수간충질간세포화지방간충질간세포대퇴변수핵세포균유일정적격활효응；골수간충질간세포대퇴변수핵세포적격활효응경강，가능경가괄합우추간반퇴행성질병적생물학치료。
Objectives: To compare the biological effects of degenerative nucleus pulposus cells when co-cul-turing two kinds of people′s mesenchymal stem cells under a non-contact system. Methods: Bone marrow mesenchymal stem cells (BMSCs), adipose mesenchymal stem cells (ADSCs) and nucleus pulposus cells (NPCs) were obtained from the same patient, then the two kinds of stem cells were co-cultured respectively with de-generative nucleus pulposus cells in Transwell six-well plates under the non-contact condition, NPCs co-cul-tivation with ADSCs as group A, and NPCs co-cultivation with BMSCs as group B, nucleus pulposus cells cultured alone as control. One week later, the total RNA of 3 groups were extracted, then the Real-Time PCR technology was used to detect the relative gene expression of type Ⅱ collagen, proteoglycan and SOX-9. Results: The relative gene expression of type Ⅱ collagen protein, proteoglycan and SOX-9 in control group was 1.03±0.28, 1.21±0.40 and 0.94±0.34 respectively, in group A was 3.49±0.55, 3.88±2.11 and 2.41±0.91 respectively, while 7.60±1.89, 6.26±2.96 and 4.55±1.88 respectively in group B, The Real-Time PCR results showed that compared with the control group, the nucleus pulposus cells of group A and B presented a sig-nificant increase of type Ⅱ collagen protein, proteoglycan and SOX-9 gene expression (P<0.05), which also showed significant difference between groups A and B(P<0.05). Conclusions: Bone marrow mesenchymal stem cells and adipose mesenchymal stem cells can both stimulate and activate the degenerative nucleus pulposus cells under the condition of noncontact co-culture; with stronger effect on the former, which may act as a better option for biological treatment for degenerative disc diseases.