中国病理生理杂志
中國病理生理雜誌
중국병리생리잡지
CHINESE JOURNAL OF PATHOPHYSIOLOGY
2014年
3期
572-576
,共5页
曾林涓%李景果%张秋波%钱辰琛%林忠%陈茵婷%黄开红
曾林涓%李景果%張鞦波%錢辰琛%林忠%陳茵婷%黃開紅
증림연%리경과%장추파%전신침%림충%진인정%황개홍
胰腺肿瘤%模型,动物%纳米微粒%RNA干扰
胰腺腫瘤%模型,動物%納米微粒%RNA榦擾
이선종류%모형,동물%납미미립%RNA간우
Pancreatic neoplasms%Models,animal%Nanoparticles%RNA interference
目的：探讨建立人胰腺癌PANC-1裸鼠移植瘤模型的最佳实验方法，并应用该模型进行载基因纳米微粒体内效应的研究。方法：将不同数量PANC-1细胞悬液接种于BALB/c （nu/nu）小鼠右侧背部皮下，当肿瘤体积达100 mm 3时尾静脉注射siRNACY 5．5纳米复合物进行活体荧光成像。此外，于尾静脉注射负载siRNA Kras纳米复合物，蛋白印迹及免疫组织化学染色法观察肿瘤组织Kras蛋白表达水平。结果：1×107 cells/300μL 接种成瘤率达100％，成瘤时间＜2周。荧光呈像及组织学检查显示载siRNA纳米微粒可靶向聚集在肿瘤组织发挥体内基因沉默效应。结论：本研究报道的人胰腺癌裸鼠移植瘤模型建立方法成瘤率达100％，成瘤时间短，是研究药物示踪和观察疗效的理想模型。
目的：探討建立人胰腺癌PANC-1裸鼠移植瘤模型的最佳實驗方法，併應用該模型進行載基因納米微粒體內效應的研究。方法：將不同數量PANC-1細胞懸液接種于BALB/c （nu/nu）小鼠右側揹部皮下，噹腫瘤體積達100 mm 3時尾靜脈註射siRNACY 5．5納米複閤物進行活體熒光成像。此外，于尾靜脈註射負載siRNA Kras納米複閤物，蛋白印跡及免疫組織化學染色法觀察腫瘤組織Kras蛋白錶達水平。結果：1×107 cells/300μL 接種成瘤率達100％，成瘤時間＜2週。熒光呈像及組織學檢查顯示載siRNA納米微粒可靶嚮聚集在腫瘤組織髮揮體內基因沉默效應。結論：本研究報道的人胰腺癌裸鼠移植瘤模型建立方法成瘤率達100％，成瘤時間短，是研究藥物示蹤和觀察療效的理想模型。
목적：탐토건립인이선암PANC-1라서이식류모형적최가실험방법，병응용해모형진행재기인납미미립체내효응적연구。방법：장불동수량PANC-1세포현액접충우BALB/c （nu/nu）소서우측배부피하，당종류체적체100 mm 3시미정맥주사siRNACY 5．5납미복합물진행활체형광성상。차외，우미정맥주사부재siRNA Kras납미복합물，단백인적급면역조직화학염색법관찰종류조직Kras단백표체수평。결과：1×107 cells/300μL 접충성류솔체100％，성류시간＜2주。형광정상급조직학검사현시재siRNA납미미립가파향취집재종류조직발휘체내기인침묵효응。결론：본연구보도적인이선암라서이식류모형건립방법성류솔체100％，성류시간단，시연구약물시종화관찰료효적이상모형。
AIM:To establish an effective and rapid method to develop transplanted subcutaneous pancreatic carcinoma by inducing PANC-1 cells into nude mice, and then use this mouse model to evaluate the tumor-homing and gene-silencing effects of siRNA-loading nanoparticles in vivo.METHODS:Different numbers of PANC-1 cells in 100 μL or 300 μL PBS were inoculated subcutaneously into the right flank of BALB /c (nu/nu) mice.When the tumor volume reached 100 mm 3 , siRNACY 5.5 nanoparticles were injected through the mouse tail vein to perform in vivo imaging assay.Be-sides, the mice were randomly divided into 3 treatment groups treated with PBS, scrambled control RNA nanoparticles and siKras nanoparticles, respectively.The protein expression of Kras was detected by Western blotting and immunohistochemi-cal staining.RESULTS:After inoculated with 1 ×10 7 PANC-1 cells in 300 μL PBS, all mice developed tumors within 2 weeks.The in vivo results showed that siRNA-loading nanoparticles accumulated in the tumor tissues and exerted gene si-lencing effect.CONCLUSION:In the present study, an effective and rapid method was established for PANC-1 cells to induce transplanted subcutaneous pancreatic carcinoma in nude mice within 2 weeks, which is suitable for in vivo imaging and treatment evaluations as a reproducible and reliable way for the further experiments .
