中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2010年
z1期
33-36
,共4页
黄连素%病周围神经病变%白介素-1β%肿瘤坏死因子-α%高敏C反应蛋白
黃連素%病週圍神經病變%白介素-1β%腫瘤壞死因子-α%高敏C反應蛋白
황련소%병주위신경병변%백개소-1β%종류배사인자-α%고민C반응단백
Berberine%Diabetic peripheral neuropathy%Interleuk-1β%Tumor necrosis factor-α%High-sensitivity C -reactive protein
目的：通过观察黄连素对糖尿病模型大鼠痛阈、坐骨神经传导速度（ NCV）及血清、坐骨神经内白介素-1β（ IL-1β）、肿瘤坏死因子-α（ TNF-α）、高敏C反应蛋白（ hs-CRP）等炎症因子的影响，探讨小檗碱治疗糖尿病周围神经病变（ DPN）的机制。方法以链脲佐菌素（ STZ）腹腔注射诱导形成DPN模型，分别以100 mg/kg或187.5 mg/kg剂量的黄连素灌胃治疗，1次/d，持续8周。用酶联免疫吸附测定（ ELISA）法分别测定各组大鼠血清及坐骨神经内IL-1β、TNF-α、hs-CRP的浓度。结果黄连素对DPN大鼠痛阈、NCV有明显改善（ P ＜0.01），与对照组比较，DPN模型组大鼠的IL-1β、TNF-α、hs-CRP含量显著增高（ P ＜0.05），黄连素治疗8周后，与DPN模型组比较IL-1β、TNF-α、hs-CRP含量明显降低（ P ＜0.05）。结论抑制细胞炎症因子IL-1β、TNF-α、hs-CRP等的释放，可能是小檗碱改善DPN的机制之一。
目的：通過觀察黃連素對糖尿病模型大鼠痛閾、坐骨神經傳導速度（ NCV）及血清、坐骨神經內白介素-1β（ IL-1β）、腫瘤壞死因子-α（ TNF-α）、高敏C反應蛋白（ hs-CRP）等炎癥因子的影響，探討小檗堿治療糖尿病週圍神經病變（ DPN）的機製。方法以鏈脲佐菌素（ STZ）腹腔註射誘導形成DPN模型，分彆以100 mg/kg或187.5 mg/kg劑量的黃連素灌胃治療，1次/d，持續8週。用酶聯免疫吸附測定（ ELISA）法分彆測定各組大鼠血清及坐骨神經內IL-1β、TNF-α、hs-CRP的濃度。結果黃連素對DPN大鼠痛閾、NCV有明顯改善（ P ＜0.01），與對照組比較，DPN模型組大鼠的IL-1β、TNF-α、hs-CRP含量顯著增高（ P ＜0.05），黃連素治療8週後，與DPN模型組比較IL-1β、TNF-α、hs-CRP含量明顯降低（ P ＜0.05）。結論抑製細胞炎癥因子IL-1β、TNF-α、hs-CRP等的釋放，可能是小檗堿改善DPN的機製之一。
목적：통과관찰황련소대당뇨병모형대서통역、좌골신경전도속도（ NCV）급혈청、좌골신경내백개소-1β（ IL-1β）、종류배사인자-α（ TNF-α）、고민C반응단백（ hs-CRP）등염증인자적영향，탐토소벽감치료당뇨병주위신경병변（ DPN）적궤제。방법이련뇨좌균소（ STZ）복강주사유도형성DPN모형，분별이100 mg/kg혹187.5 mg/kg제량적황련소관위치료，1차/d，지속8주。용매련면역흡부측정（ ELISA）법분별측정각조대서혈청급좌골신경내IL-1β、TNF-α、hs-CRP적농도。결과황련소대DPN대서통역、NCV유명현개선（ P ＜0.01），여대조조비교，DPN모형조대서적IL-1β、TNF-α、hs-CRP함량현저증고（ P ＜0.05），황련소치료8주후，여DPN모형조비교IL-1β、TNF-α、hs-CRP함량명현강저（ P ＜0.05）。결론억제세포염증인자IL-1β、TNF-α、hs-CRP등적석방，가능시소벽감개선DPN적궤제지일。
Objective To investigate the effects of berberine on interleuk-1β( IL-1β) and tumor necrosis factor-α( TNF-α) and high-sensitivity C-reactive protein ( hs-CRP ) in diabetes rats induced by Streptozotocin ( STZ) , to explore the possible mechanisms of berberine on diabetic peripheral neuropathy . Methods Adult male SD rats were randomly divided into 4 groups:normal group , diabetic peripheral neu-ropathy group, berberine (100 mg/kg) group, berberine (187.5 mg/kg) group.The diabetic rat modes were i.p.injected with 1%STZ for two weeks, the rats were treated for 8 weeks respectively with berber-ine.The contents of IL-1βand TNF-αand hs-CRP in the serum and sciatic nerve were determined respec-tively by ELISA .Results The concentration of IL-1βand TNF-αand hs-CRP dropped obviously compared to DPN group ( P <0.05 ) .Conclusion It is suggested that berberine could possess the functions of de-creasing inflammatory mediator , antagonizing inflammatory reaction .Its therapeutic mechanism might be correlated with the improvement of inflammatory condition of diabetic rats ,and the promotion of sensitivity of insulin signal transduction in target tissues .
