中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2010年
z1期
19-21
,共3页
生殖器疱疹%白细胞介素2%转录因子STAT%T细胞
生殖器皰疹%白細胞介素2%轉錄因子STAT%T細胞
생식기포진%백세포개소2%전록인자STAT%T세포
Herpes genital%Interleukin 2%Signal transducer and activator of transcription 5%T cell
目的：探讨皮下注射白细胞介素2后生殖器疱疹患者T细胞中磷酸化信号转导和转录激活因子5（ Signal transducer and activator of transcription 5，STAT5）的表达和STAT5的DNA结合活性。方法共6例复发性生殖器疱疹患者进入本研究，白细胞介素2皮下注射28 d后，采血，分离T细胞，采用蛋白免疫印迹技术和凝胶滞留法（ Electrophoretic mobility shift assay ， EMSA）检测了外周血T细胞中磷酸化STAT5的表达和STAT5的DNA结合活性，并对其进行了定量分析。结果经白细胞介素2治疗后第28天，可见生殖器疱疹患者外周血T细胞核蛋白中磷酸化STAT5的表达是未经治疗时的1.8～2.7倍；治疗后STAT5的DNA结合活性增高2.3～3.4倍，治疗后与治疗前之间的差异有统计学意义（ t ＝10.6， P ＜0.01）。结论白细胞介素2皮下注射后生殖器疱疹患者T细胞中磷酸化STAT5的表达和STAT5的DNA结合活性显著增高，提示STAT5是白细胞介素2治疗的重要信号介质，并且STAT5的激活可提示患者是否对白细胞介素2治疗有反应。为临床应用白细胞介素2治疗生殖器疱疹提供了临床理论依据。
目的：探討皮下註射白細胞介素2後生殖器皰疹患者T細胞中燐痠化信號轉導和轉錄激活因子5（ Signal transducer and activator of transcription 5，STAT5）的錶達和STAT5的DNA結閤活性。方法共6例複髮性生殖器皰疹患者進入本研究，白細胞介素2皮下註射28 d後，採血，分離T細胞，採用蛋白免疫印跡技術和凝膠滯留法（ Electrophoretic mobility shift assay ， EMSA）檢測瞭外週血T細胞中燐痠化STAT5的錶達和STAT5的DNA結閤活性，併對其進行瞭定量分析。結果經白細胞介素2治療後第28天，可見生殖器皰疹患者外週血T細胞覈蛋白中燐痠化STAT5的錶達是未經治療時的1.8～2.7倍；治療後STAT5的DNA結閤活性增高2.3～3.4倍，治療後與治療前之間的差異有統計學意義（ t ＝10.6， P ＜0.01）。結論白細胞介素2皮下註射後生殖器皰疹患者T細胞中燐痠化STAT5的錶達和STAT5的DNA結閤活性顯著增高，提示STAT5是白細胞介素2治療的重要信號介質，併且STAT5的激活可提示患者是否對白細胞介素2治療有反應。為臨床應用白細胞介素2治療生殖器皰疹提供瞭臨床理論依據。
목적：탐토피하주사백세포개소2후생식기포진환자T세포중린산화신호전도화전록격활인자5（ Signal transducer and activator of transcription 5，STAT5）적표체화STAT5적DNA결합활성。방법공6례복발성생식기포진환자진입본연구，백세포개소2피하주사28 d후，채혈，분리T세포，채용단백면역인적기술화응효체류법（ Electrophoretic mobility shift assay ， EMSA）검측료외주혈T세포중린산화STAT5적표체화STAT5적DNA결합활성，병대기진행료정량분석。결과경백세포개소2치료후제28천，가견생식기포진환자외주혈T세포핵단백중린산화STAT5적표체시미경치료시적1.8～2.7배；치료후STAT5적DNA결합활성증고2.3～3.4배，치료후여치료전지간적차이유통계학의의（ t ＝10.6， P ＜0.01）。결론백세포개소2피하주사후생식기포진환자T세포중린산화STAT5적표체화STAT5적DNA결합활성현저증고，제시STAT5시백세포개소2치료적중요신호개질，병차STAT5적격활가제시환자시부대백세포개소2치료유반응。위림상응용백세포개소2치료생식기포진제공료림상이론의거。
Objective To observe the expression of phosphorylation of signal transducer and activa -tor of transcription 5 (STAT5) protein and the DNA-binding activity of STAT5 after interleukin 2 therapy for genital herpes .Methods Six patients with recurrent genital herpes were included in this study .CD4 +T cells from patients who underwent interleukin 2 therapy were analyzed for changes in STAT 5 signaling.None had been treated with corticosteroid and immunosuppressive agents .Phosphorylation of STAT 5 was detected in the T cells using Western blot analysis .Electrophoretic mobility shift assay ( EMSA) was carried out to detect the DNA-biding activity of STAT5.Results The expressions of phosphorylated STAT 5 in T cells de-rived from patients with genital herpes 28 days after interleukin treatment were 1.8~2.7 fold to that of be-fore the treatment was given .STAT5 DNA-binding activity in T cells derived form patients with genital her-pes who had received the treatment was 2.3~3.4 fold increased compared to that of before the initiating of interleukin 2 treatment.The differences between the before and after interleukin 2 treatment were statistical-ly significant( t =10.6, P <0.05).Conclusion This study indicates that STAT5 may be an important signaling mediator of interleukin 2 therapies , and that STAT5 activation may predict response to this treat-ment .
