CAJ | 학술논문

目的：单克隆抗体检测麻疹病毒抗原与一步法RT-PCR检测麻疹病毒RNA在实验室诊断中的对比。方法收集疑似麻疹患者咽拭子标本。出疹3 d后血清抗麻疹病毒IgM抗体阳性者咽拭子纳入对比试验。用荧光标记的鼠抗麻疹病毒单克隆抗体检测标本中麻疹病毒抗原成份；用一步法RT-PCR结合Taqman技术检测麻疹病毒RNA成份。结果82份疑似麻疹患者抗麻疹病毒IgM抗体阳性率81.71%（67/82）。67例确诊病例标本单克隆抗体间接免疫荧光阳性率88.06%（59/67）；一步法RT-PCR结果阳性率98.51%（66/67）。结论麻疹病毒RNA的一步法RT-PCR结合Taqman检测敏感性高于其抗原的间接免疫荧光检测。
목적：단극륭항체검측마진병독항원여일보법RT-PCR검측마진병독RNA재실험실진단중적대비。방법수집의사마진환자인식자표본。출진3 d후혈청항마진병독IgM항체양성자인식자납입대비시험。용형광표기적서항마진병독단극륭항체검측표본중마진병독항원성빈；용일보법RT-PCR결합Taqman기술검측마진병독RNA성빈。결과82빈의사마진환자항마진병독IgM항체양성솔81.71%（67/82）。67례학진병례표본단극륭항체간접면역형광양성솔88.06%（59/67）；일보법RT-PCR결과양성솔98.51%（66/67）。결론마진병독RNA적일보법RT-PCR결합Taqman검측민감성고우기항원적간접면역형광검측。
Objective To contrast the effective rate between measles virus antigen detection by monoclonal antibody and RNA detection by one step RT-PCR. Methods The throat swab samples were collected from every suspected measle patient. Then the measles virus antigen using monoclonal antibody were detected while the measles virus RNA detected by one step RT-PCR after the patients’ anti-measle viral IgM antibody were deifnited positive.Results Total of 67 plasma cases’ anti-measle viral IgM antibody among 82 patiens were positive. In the 67 cases, monoclonal antibody indirect immunolfuorescence positive ratio was 88.06% (59/67). One step RT-PCR positive ratio was 98.51% (66/67).Conclusions One step RT-PCR detectection method was surpass to the monoclonal antibody detectetion method for the diagnosis of the measles infection on the aboratory level.