中华老年口腔医学杂志
中華老年口腔醫學雜誌
중화노년구강의학잡지
CHINESE JOURNAL OF GERIATRIC DENTISTRY
2014年
3期
134-139
,共6页
陈丽虹%舒珊%陈丽红%韦曦
陳麗虹%舒珊%陳麗紅%韋晞
진려홍%서산%진려홍%위희
锌指E盒结合同源框2%TGF-β1%牙髓细胞%成牙本质细胞
鋅指E盒結閤同源框2%TGF-β1%牙髓細胞%成牙本質細胞
자지E합결합동원광2%TGF-β1%아수세포%성아본질세포
zinc-finger E-box binding homeobox 2/Smad-interacting protein1%TGF-β1%Human dental pulp cells%odontoblast
目的：检测锌指E盒结合同源框2(ZEB2)在人牙髓组织和细胞中的表达，并初步探讨其意义。方法：制作牙髓组织石蜡切片，荧光原位杂交技术(FISH)检测 ZEB2的表达；实时荧光定量聚合酶链反应(RT-qPCR)检测正常及TGF-β1刺激下人牙髓细胞(hDPCs)中ZEB2 mRNA表达水平；制作hDPCs细胞爬片， FISH检测ZEB2的表达。结果： FISH结果显示ZEB2在牙髓组织中主要表达于成牙本质细胞层，在牙髓细胞呈弱阳性表达。 RT-qPCR结果显示TGF-β1的作用促进ZEB2的表达，且具有浓度和时间依赖性，5ng/ml TGF-β1刺激ZEB2表达达最大(P<0.05)；5ng/ml TGF-β1刺激后， ZEB2 mRNA在24h内表达逐渐上调，24h达峰值(P<0.05)。 FISH结果示ZEB2在正常hDPCs胞质和胞核均呈弱阳性表达， TGF-β1刺激24h后ZEB2表达增强，胞核表达明显。结论： ZEB2在牙髓组织中主要表达于成牙本质细胞层，正常hDPCs中弱阳性表达，而TGF-β1可促进hDPCs中ZEB2表达，提示ZEB2可能通过参与TGF-β1信号通路调控hDPCs的成牙本质向分化过程。
目的：檢測鋅指E盒結閤同源框2(ZEB2)在人牙髓組織和細胞中的錶達，併初步探討其意義。方法：製作牙髓組織石蠟切片，熒光原位雜交技術(FISH)檢測 ZEB2的錶達；實時熒光定量聚閤酶鏈反應(RT-qPCR)檢測正常及TGF-β1刺激下人牙髓細胞(hDPCs)中ZEB2 mRNA錶達水平；製作hDPCs細胞爬片， FISH檢測ZEB2的錶達。結果： FISH結果顯示ZEB2在牙髓組織中主要錶達于成牙本質細胞層，在牙髓細胞呈弱暘性錶達。 RT-qPCR結果顯示TGF-β1的作用促進ZEB2的錶達，且具有濃度和時間依賴性，5ng/ml TGF-β1刺激ZEB2錶達達最大(P<0.05)；5ng/ml TGF-β1刺激後， ZEB2 mRNA在24h內錶達逐漸上調，24h達峰值(P<0.05)。 FISH結果示ZEB2在正常hDPCs胞質和胞覈均呈弱暘性錶達， TGF-β1刺激24h後ZEB2錶達增彊，胞覈錶達明顯。結論： ZEB2在牙髓組織中主要錶達于成牙本質細胞層，正常hDPCs中弱暘性錶達，而TGF-β1可促進hDPCs中ZEB2錶達，提示ZEB2可能通過參與TGF-β1信號通路調控hDPCs的成牙本質嚮分化過程。
목적：검측자지E합결합동원광2(ZEB2)재인아수조직화세포중적표체，병초보탐토기의의。방법：제작아수조직석사절편，형광원위잡교기술(FISH)검측 ZEB2적표체；실시형광정량취합매련반응(RT-qPCR)검측정상급TGF-β1자격하인아수세포(hDPCs)중ZEB2 mRNA표체수평；제작hDPCs세포파편， FISH검측ZEB2적표체。결과： FISH결과현시ZEB2재아수조직중주요표체우성아본질세포층，재아수세포정약양성표체。 RT-qPCR결과현시TGF-β1적작용촉진ZEB2적표체，차구유농도화시간의뢰성，5ng/ml TGF-β1자격ZEB2표체체최대(P<0.05)；5ng/ml TGF-β1자격후， ZEB2 mRNA재24h내표체축점상조，24h체봉치(P<0.05)。 FISH결과시ZEB2재정상hDPCs포질화포핵균정약양성표체， TGF-β1자격24h후ZEB2표체증강，포핵표체명현。결론： ZEB2재아수조직중주요표체우성아본질세포층，정상hDPCs중약양성표체，이TGF-β1가촉진hDPCs중ZEB2표체，제시ZEB2가능통과삼여TGF-β1신호통로조공hDPCs적성아본질향분화과정。
Objective:To investigate the expression of zinc-finger E-box binding homeobox 2 in human dental pulp tissue and cells. Methods: Using fluorescence in situ hybridization (FISH) to test and verify the expression of ZEB2 in nomal dental pulp tissue. The expression levels of ZEB2 in normal hDPCs and hDPCs stimulated by TGF-β1 were detected by real-time fluorescentquantitative PCR (RT-qPCR) and FISH. Results: FISH array of pulp tissue showed that ZEB2 was mainly expressed in odontoblasts. The expression of ZEB2 was weakly positive in the nucleus and cytoplasm of normal hDPCs. TGF-β1 can promote the expression of ZEB2. The expression of ZEB2 was up-regulated obviously when hDPCs were stimulated by 5ng/ml TGF-β1. RT-PCR showed that ZEB2 mRNA tending to increase from 0h to 24h. There was a down trend a over time (P<0.05). After stimulated by TGF- β1 for 24h, the expression of ZEB2 was strong positive, especially in nuclear. Conclusion:ZEB2 was mainly expressed in odontoblasts in normal dental pulp tissue. The expression level of ZEB2 was positively correlated with TGF-β1 which suggested that ZEB2 involved in TGF-β1 signaling pathway and may play an role of regulation in the process of hDPCs differentiated into odontoblast.
