CAJ | 학술논문

目的：探讨SIAH2蛋白在肝细胞癌（肝癌）发生、发展中的作用。方法取对数生长期的人肝癌细胞Bel-7404，分别将空载体小干扰核糖核酸（siRNA）及SIAH2 siRNA转染至人肝癌细胞Bel-7404中，将转染后细胞分为control-siRNA组和SIAH2-siRNA组。以β-肌动蛋白（β-actin）为对照，采用蛋白质印迹法检测两组人肝癌细胞Bel-7404的SIAH2蛋白表达情况。采用噻唑蓝法检测两组细胞增殖情况，采用细胞划痕实验观察两组细胞迁移情况，采用Transwell实验观察两组细胞侵袭情况。两组间的数据比较采用t检验。结果 control-siRNA组和SIAH2-siRNA组的SIAH2蛋白平均相对表达量分别为0.71±0.02、0.33±0.01。与control-siRNA组相比，SIAH2-siRNA组的SIAH2蛋白相对表达量明显减少（t=-4.629，P<0.05），增殖速度亦明显下降。control-siRNA组的细胞迁移率为（45.3±0.4）%，SIAH2-siRNA组为（14.3±0.4）%，SIAH2-siRNA组明显低于control-siRNA组（t=-3.689，P<0.05）。control-siRNA组的穿膜细胞数为（563±10）个，SIAH2-siRNA组为（122±7）个，SIAH2-siRNA组明显少于control-siRNA组（t=-3.428，P<0.05）。结论 SIAH2蛋白能促进肝癌细胞增殖、迁移、侵袭，从而加速肿瘤的发生、发展。
목적：탐토SIAH2단백재간세포암（간암）발생、발전중적작용。방법취대수생장기적인간암세포Bel-7404，분별장공재체소간우핵당핵산（siRNA）급SIAH2 siRNA전염지인간암세포Bel-7404중，장전염후세포분위control-siRNA조화SIAH2-siRNA조。이β-기동단백（β-actin）위대조，채용단백질인적법검측량조인간암세포Bel-7404적SIAH2단백표체정황。채용새서람법검측량조세포증식정황，채용세포화흔실험관찰량조세포천이정황，채용Transwell실험관찰량조세포침습정황。량조간적수거비교채용t검험。결과 control-siRNA조화SIAH2-siRNA조적SIAH2단백평균상대표체량분별위0.71±0.02、0.33±0.01。여control-siRNA조상비，SIAH2-siRNA조적SIAH2단백상대표체량명현감소（t=-4.629，P<0.05），증식속도역명현하강。control-siRNA조적세포천이솔위（45.3±0.4）%，SIAH2-siRNA조위（14.3±0.4）%，SIAH2-siRNA조명현저우control-siRNA조（t=-3.689，P<0.05）。control-siRNA조적천막세포수위（563±10）개，SIAH2-siRNA조위（122±7）개，SIAH2-siRNA조명현소우control-siRNA조（t=-3.428，P<0.05）。결론 SIAH2단백능촉진간암세포증식、천이、침습，종이가속종류적발생、발전。
Objective To investigate the role of SIAH2 protein in the occurrence and development of hepatocellular carcinoma (HCC). Methods Human HCC Bel-7404 cells in logarithmic growth phase were inoculated. Empty carrier small interference ribonucleic acid (siRNA) and SIAH2 siRNA were transfected in human HCC Bel-7404 cells. Then the cells were divided into 2 groups:control-siRNA group and SIAH2-siRNA group. Theβ-actin was taken as control, and the expression of SIAH2 protein in human HCC Bel-7404 cells of 2 groups was detected by Western Blot. The proliferation of cells in 2 groups was detected by methyl thiazolyl tetrazolium (MTT) assay. Cell migration in 2 groups was observed by cell scratch test. Cell invasion in 2 groups was observed by Transwell assay. The data in 2 groups were compared using t test. Results The average relative expression of SIAN2 in control-siRNA and SIAH2-siRNA group were 0.71±0.02, 0.33±0.01 respectively. The expression of SIAN2 in SIAH2-siRNA group decreased obviously compared with that in control-siRNA group (t=-4.629, P<0.05). The proliferation rate in SIAH2-siRNA group also decreased obviously. The cell migration rate in SIAH2-siRNA group[(14.3±0.4)%] was significantly lower than that in control-siRNA group [(45.3±0.4)%]( t=-3.689, P<0.05). The membrane permeating cell count in SIAH2-siRNA group (122±7) was signiifcantly less than that in control-siRNA group (563±10) (t=-3.428, P<0.05). Conclusion SIAH2 protein can promote the proliferation, migration and invasion of HCC cells and thus accelerate the occurrence and development of HCC.