山西中医学院学报
山西中醫學院學報
산서중의학원학보
JOURNAL OF SHANXI COLLEGE OF TRADITIONAL CHINESE MEDICINE
2014年
3期
37-38,41
,共3页
姜辉%刘健%孟楣%桂洁
薑輝%劉健%孟楣%桂潔
강휘%류건%맹미%계길
五味温通除痹胶囊%UPLC%黄芩苷%含量测定
五味溫通除痺膠囊%UPLC%黃芩苷%含量測定
오미온통제비효낭%UPLC%황금감%함량측정
Wuwei Wentong Chubi capsule%UPLC%baicalin%content determination
目的：建立五味温通除痹胶囊中黄芩苷的含量测定方法。方法：采用超高液相色谱法，以Acquity UPLC BEH C18柱（100 mm×2.1 mm，1.7μm）为色谱柱，乙腈-0.1%磷酸（25∶75）为流动相；流速：0.25 mL/min；柱温：30℃；检测波长280 nm。结果：黄芩苷在0.0955 mg~0.955 mg范围内呈良好的线性关系，平均回收率为99.21%，RSD=1.16%。结论：该法准确可靠、重复性好，可用于该制剂中黄芩苷的含量测定。
目的：建立五味溫通除痺膠囊中黃芩苷的含量測定方法。方法：採用超高液相色譜法，以Acquity UPLC BEH C18柱（100 mm×2.1 mm，1.7μm）為色譜柱，乙腈-0.1%燐痠（25∶75）為流動相；流速：0.25 mL/min；柱溫：30℃；檢測波長280 nm。結果：黃芩苷在0.0955 mg~0.955 mg範圍內呈良好的線性關繫，平均迴收率為99.21%，RSD=1.16%。結論：該法準確可靠、重複性好，可用于該製劑中黃芩苷的含量測定。
목적：건립오미온통제비효낭중황금감적함량측정방법。방법：채용초고액상색보법，이Acquity UPLC BEH C18주（100 mm×2.1 mm，1.7μm）위색보주，을정-0.1%린산（25∶75）위류동상；류속：0.25 mL/min；주온：30℃；검측파장280 nm。결과：황금감재0.0955 mg~0.955 mg범위내정량호적선성관계，평균회수솔위99.21%，RSD=1.16%。결론：해법준학가고、중복성호，가용우해제제중황금감적함량측정。
Objective:To establish a method for content determination of baicalin in Wuwei Wentong Chubi capsule by UPLC. Methods:UPLC method was performed on the acquity UPLC BEH C18(100 mm×2.1 mm,1.7μm)column with ace-tonitrile-0.1%phosphoric acid(25∶75)as mobile phase and the flow rate was 0.25 mL/min. Detection wavelength was set at 280 nm and the column temperature was 30 ℃. Results:The linear ranger of baicalin was 0.095 5 μg~0.955 0 μg. Aver-age recovery rate was 99.21% and RSD was 1.16%. Conclusions:The method is accurate,reliable and practical,and can be used for the quality control of Wuwei Wentong Chubi capsule.
