CAJ | 학술논문

目的：在转染并稳定表达Cx32的HeLa细胞上，观察黄芩素对Cx32组成的GJIC及Cx32蛋白表达水平的影响。方法：细胞接种荧光示踪法观察不同浓度黄芩素对GJIC的影响；细胞免疫荧光法观察黄芩素在影响GJIC功能浓度范围内对Cx32蛋白表达的影响。结果：黄芩素（2.5μmol/L，5μmol/L，10μmol/L）增强Cx32组成的GJIC功能的荧光渗透率为10.04%，22.5%和35.33%；黄芩素（2.5μmol/L，5μmol/L，10μmol/L）增强组成GJIC的Cx32蛋白表达的荧光强度为（27.12±0.30）%，（56.31±1.02）%和（83.47±1.85）%。结论：黄芩素可增强缝隙连接蛋白Cx32组成的GJIC功能，其机制可能与增加Cx32蛋白表达水平有关。
목적：재전염병은정표체Cx32적HeLa세포상，관찰황금소대Cx32조성적GJIC급Cx32단백표체수평적영향。방법：세포접충형광시종법관찰불동농도황금소대GJIC적영향；세포면역형광법관찰황금소재영향GJIC공능농도범위내대Cx32단백표체적영향。결과：황금소（2.5μmol/L，5μmol/L，10μmol/L）증강Cx32조성적GJIC공능적형광삼투솔위10.04%，22.5%화35.33%；황금소（2.5μmol/L，5μmol/L，10μmol/L）증강조성GJIC적Cx32단백표체적형광강도위（27.12±0.30）%，（56.31±1.02）%화（83.47±1.85）%。결론：황금소가증강봉극련접단백Cx32조성적GJIC공능，기궤제가능여증가Cx32단백표체수평유관。
Objective:To investigat the effect of baicalien on gap junction intercellular communication and the expression of Cx32 protein in Cx32-transfected HeLa cells. Methods:The function of GJIC was determined by"parachute"dye-coupling assay. Immunofluorescence assay was used to detect the expression of Cx32 protein located in the membrane of HeLa cells. Results:"Parachute" assay results showed that the intercellular dye coupling through gap junction composed of Cx32 increased by 10.04%,22.5%and 35.33% when the cells exposed to ellagic acid of 2.5 μmol/L,5 μmol/L and 10 μmol/L. Immunofluorescence assay results showed that fluorescence intensity for the expression of Cx32 protein located in membrane could be enhanced by (27.12±0.30)%,(56.31±1.02)%and (83.47±1.85)%when cells were exposed to baicalein of 2.5μmol/L,5 μmol/L and 10 μmol/L. Conclusion:Baicalein can enhance the function of gap junction in Cx32-transfected HeLa cells,probably through increasing the Cx32 expression.