CAJ | 학술논문

【目的】傅里叶变换红外光谱( fourier transform infrared spectroscopy, FTIR)是一种基于化合物中官能团和极性键振动的结构分析技术。本文利用傅立叶变换红外光谱仪检测缺锌和正常供锌玉米植株不同器官的组分变化,同时比较两个玉米品种植株不同部位的生物量和锌含量,以期为缺锌影响玉米生长与生理代谢的机理研究提供参考。【方法】选取农大108和郑单958两个玉米品种,利用营养液培养方式,设置缺锌和正常处理。1)当玉米出现缺锌症状后,将地上部和根系分开,测量株高和根长,烘干至恒重测干重。2)烘干至恒重的植株样品用HNO3-HClO4(3：1)消煮,原子吸收分光光度计(型号WFX-120C,北京瑞利分析仪器公司)测定消煮液中锌浓度,计算植株中锌含量和锌积累量。3)收获玉米根系放入FAA固定液(70%酒精：38%甲醛：乙酸体积=90：5：5)中,利用扫描仪(EsponV700)扫描根系样品获取数字化图像,利用WinRHIZO根系分析软件(Regent Instruments Inc., Canada)对图像进行分析,获得根长、根面积、根体积等指标。4)取玉米根、茎、叶部分烘干样品,磨碎过0.2 mm筛,采用溴化钾压片法,利用傅立叶变换红外光谱仪(VERTEX 70,Bruker)检测不同部位的光谱特性,OPUS 6.5软件采集数据并进行基线校正。【结果】缺锌胁迫下,植株地上部锌含量明显下降,低于临界水平(20μg/g),生物量降低；缺锌根系面积与体积变小,总根长变小。用缺锌与施锌植株生物量比来表征玉米对缺锌敏感性,品种农大108较郑单958对缺锌更为敏感。缺锌玉米根系和叶片FTIR谱在波数3410、2920、1650、1380、1055 cm-1附近处透过率较高,茎FTIR谱在这些波数处透过率较低,表明缺锌导致根系和叶片中碳水化合物、脂类、蛋白质及核酸含量下降,而在茎中有所积累。农大108植株中各组分变化受缺锌影响较大。【结论】缺锌导致玉米植株生长受抑,利用FTIR技术研究发现缺锌植株中碳水化合物、脂类、蛋白质及核酸组分发生变化,农大108植株中各组分变化受缺锌影响较大,品种农大108可能较郑单958对缺锌更为敏感。【目的】傅裏葉變換紅外光譜( fourier transform infrared spectroscopy, FTIR)是一種基于化閤物中官能糰和極性鍵振動的結構分析技術。本文利用傅立葉變換紅外光譜儀檢測缺鋅和正常供鋅玉米植株不同器官的組分變化,同時比較兩箇玉米品種植株不同部位的生物量和鋅含量,以期為缺鋅影響玉米生長與生理代謝的機理研究提供參攷。【方法】選取農大108和鄭單958兩箇玉米品種,利用營養液培養方式,設置缺鋅和正常處理。1)噹玉米齣現缺鋅癥狀後,將地上部和根繫分開,測量株高和根長,烘榦至恆重測榦重。2)烘榦至恆重的植株樣品用HNO3-HClO4(3：1)消煮,原子吸收分光光度計(型號WFX-120C,北京瑞利分析儀器公司)測定消煮液中鋅濃度,計算植株中鋅含量和鋅積纍量。3)收穫玉米根繫放入FAA固定液(70%酒精：38%甲醛：乙痠體積=90：5：5)中,利用掃描儀(EsponV700)掃描根繫樣品穫取數字化圖像,利用WinRHIZO根繫分析軟件(Regent Instruments Inc., Canada)對圖像進行分析,穫得根長、根麵積、根體積等指標。4)取玉米根、莖、葉部分烘榦樣品,磨碎過0.2 mm篩,採用溴化鉀壓片法,利用傅立葉變換紅外光譜儀(VERTEX 70,Bruker)檢測不同部位的光譜特性,OPUS 6.5軟件採集數據併進行基線校正。【結果】缺鋅脅迫下,植株地上部鋅含量明顯下降,低于臨界水平(20μg/g),生物量降低；缺鋅根繫麵積與體積變小,總根長變小。用缺鋅與施鋅植株生物量比來錶徵玉米對缺鋅敏感性,品種農大108較鄭單958對缺鋅更為敏感。缺鋅玉米根繫和葉片FTIR譜在波數3410、2920、1650、1380、1055 cm-1附近處透過率較高,莖FTIR譜在這些波數處透過率較低,錶明缺鋅導緻根繫和葉片中碳水化閤物、脂類、蛋白質及覈痠含量下降,而在莖中有所積纍。農大108植株中各組分變化受缺鋅影響較大。【結論】缺鋅導緻玉米植株生長受抑,利用FTIR技術研究髮現缺鋅植株中碳水化閤物、脂類、蛋白質及覈痠組分髮生變化,農大108植株中各組分變化受缺鋅影響較大,品種農大108可能較鄭單958對缺鋅更為敏感。
【목적】부리협변환홍외광보( fourier transform infrared spectroscopy, FTIR)시일충기우화합물중관능단화겁성건진동적결구분석기술。본문이용부립협변환홍외광보의검측결자화정상공자옥미식주불동기관적조분변화,동시비교량개옥미품충식주불동부위적생물량화자함량,이기위결자영향옥미생장여생리대사적궤리연구제공삼고。【방법】선취농대108화정단958량개옥미품충,이용영양액배양방식,설치결자화정상처리。1)당옥미출현결자증상후,장지상부화근계분개,측량주고화근장,홍간지항중측간중。2)홍간지항중적식주양품용HNO3-HClO4(3：1)소자,원자흡수분광광도계(형호WFX-120C,북경서리분석의기공사)측정소자액중자농도,계산식주중자함량화자적루량。3)수획옥미근계방입FAA고정액(70%주정：38%갑철：을산체적=90：5：5)중,이용소묘의(EsponV700)소묘근계양품획취수자화도상,이용WinRHIZO근계분석연건(Regent Instruments Inc., Canada)대도상진행분석,획득근장、근면적、근체적등지표。4)취옥미근、경、협부분홍간양품,마쇄과0.2 mm사,채용추화갑압편법,이용부립협변환홍외광보의(VERTEX 70,Bruker)검측불동부위적광보특성,OPUS 6.5연건채집수거병진행기선교정。【결과】결자협박하,식주지상부자함량명현하강,저우림계수평(20μg/g),생물량강저；결자근계면적여체적변소,총근장변소。용결자여시자식주생물량비래표정옥미대결자민감성,품충농대108교정단958대결자경위민감。결자옥미근계화협편FTIR보재파수3410、2920、1650、1380、1055 cm-1부근처투과솔교고,경FTIR보재저사파수처투과솔교저,표명결자도치근계화협편중탄수화합물、지류、단백질급핵산함량하강,이재경중유소적루。농대108식주중각조분변화수결자영향교대。【결론】결자도치옥미식주생장수억,이용FTIR기술연구발현결자식주중탄수화합물、지류、단백질급핵산조분발생변화,농대108식주중각조분변화수결자영향교대,품충농대108가능교정단958대결자경위민감。
[Objectives]Fourier transform infrared spectroscopy ( FTIR) is a structural analysis technique based on the vibrations of functional group and polar bond in chemical components. The objective of this study was to reveal the component changes in different organs of maize plants with and without zinc( Zn) application using FTIR. It would provide some physiological evidences of maize plants subjected to Zn deficiency stress.[Methods]Two maize cultivars, Nongda108 and Zhengdan958, were chosen as tested crops. Hydroponic culture experiments were conducted with 0 and 1. 0 μmol/L Zn treatments. The plant shoot and root biomass were weighted. Dry plant samples were digested with HNO3 -HClO4 ( 3:1 ) for Zn determination by atomic absorption spectrophotometer. Root samples were stored in FAA solution ( 70% alcohol:38% formaldehyde:acetic acid = 90 :5:5 parts by volume) prior to measurements. The root systems were then digitized with the EsponV700 scanner at 300 dpi resolution for further analysis. The total root length and root volume were measured with WinRHIZO root analysis software ( Regent Instruments Inc. , Canada) . Dry samples of roots, stems and leaves were ground to through 0. 2 mm sieve. A potassium bromide tablet method was used to detect spectral characteristics of different plant parts with FTIR (VERTEX 70, Bruker) and data were collected and analyzed by the OPUS 6. 5 software. [Results]The results show that Zn concentrations in maize plants without Zn application are below the critical level of 20 μg/g, and the Zn deficiency stress significantly decreases shoot dry matter weight, root surface, root volume and total root length. The shoot biomass and root length of maize cultivar, Nongda108, are less than those of Zhengdan95 under the Zn deficiency, which indicates Nongda108 might be more sensitive to Zn deficiency. Compared with the 1. 0μmol/L Zn treatment, plants with Zn deficiency show higher transmittance at peaks of 3410, 2920, 1650, 1380 and 1055 cm-1 in FTIR spectra of roots and leaves and lower transmittance in stems, which indicates the sugar, lipid and protein contents in roots and leaves are decreased, and the contents in stems are increased under the Zn deficiency stress. [Conclusions]Zn deficiency has a great negative influence on plant growth and results in changes of organic components contents, including starch, lipids, protein, and nucleic acids in different parts of plants based on FTIR determinations. Nongda108 might be more sensitive to Zn deficiency compared with Zhengdan958.