CAJ | 학술논문

目的：比较四氯化碳（ carbon tetrachloride ，CCl4）诱导小鼠急性、亚急性、慢性肝损伤模型的差异，探讨稳定、可靠的小鼠肝损伤模型复制方法。方法：以CCl4油溶液（油为食用植物油）为材料，分别通过腹腔注射、皮下注射、灌胃等给药方式，固定给药时间，观察血清谷草转氨酶（ AST）、谷丙转氨酶（ ALT）活性及肝组织细胞形态的变化，考察肝损伤模型复制的的稳定性、可靠性。结果：腹腔注射不同浓度的CCl4均可产生急性肝损伤，使血清ALT明显升高，但只有高剂量才能升高AST；皮下注射不同浓度的CCl4均可产生亚急性肝损伤，CCl4中剂量组不仅可使血清ALT明显升高，还能升高AST；灌胃不同浓度的CCl4均可产生慢性肝损伤，CCl4各剂量组不仅可使血清ALT明显升高，还能升高AST，作用稳定，且呈浓度依赖性。肝组织病理切片显示急性、亚急性肝损伤模型肝组织坏死严重，组间没有明显区别；而慢性肝损伤模型出现肝细胞变性、部位肝组织坏死，浓度间呈现不同趋势。结论：CCl4诱导的慢性肝损伤模型复制稳定、病理特征明显，更有利于用于考察药物的作用及机制。
목적：비교사록화탄（ carbon tetrachloride ，CCl4）유도소서급성、아급성、만성간손상모형적차이，탐토은정、가고적소서간손상모형복제방법。방법：이CCl4유용액（유위식용식물유）위재료，분별통과복강주사、피하주사、관위등급약방식，고정급약시간，관찰혈청곡초전안매（ AST）、곡병전안매（ ALT）활성급간조직세포형태적변화，고찰간손상모형복제적적은정성、가고성。결과：복강주사불동농도적CCl4균가산생급성간손상，사혈청ALT명현승고，단지유고제량재능승고AST；피하주사불동농도적CCl4균가산생아급성간손상，CCl4중제량조불부가사혈청ALT명현승고，환능승고AST；관위불동농도적CCl4균가산생만성간손상，CCl4각제량조불부가사혈청ALT명현승고，환능승고AST，작용은정，차정농도의뢰성。간조직병리절편현시급성、아급성간손상모형간조직배사엄중，조간몰유명현구별；이만성간손상모형출현간세포변성、부위간조직배사，농도간정현불동추세。결론：CCl4유도적만성간손상모형복제은정、병리특정명현，경유리우용우고찰약물적작용급궤제。
Objective:To compare the difference among acute、subacute、chronic liver injury of mice induced by CCl 4 , exploring a sta-ble、reliable method to create a liver injury model in mice .Methods:The model of liver injury was made by CCl 4 oil solution with subcu-taneous injection、intraperitoneal injection or by gavage , and fixed the delivery time .Observation of the changes of plasma ALT、AST and liver slices , to investigate stability and reliability of the method .Results:All of different concentrations of CCl 4 can produce acute , subacute , chronic liver injury , and in aspects of acute liver injury , ALT increased significantly , the level of AST elevated only in high dose groups .As for subacute liver injury , middle dose can increase both ALT and AST obviously .Concerning the chronic liver injury , plasma ALT and AST in all dose groups were markedly higher than those in the control group in a concentration -dependent manner . Conclusion:The model of chronic liver injury induced by CCl 4 , was the most stable , and clinicopathological features were obvious , so it was beneficial to investigate the roles and mechanism of drugs .