江西中医药大学学报
江西中醫藥大學學報
강서중의약대학학보
Journal of Jiangxi University of Traditional Chinese Medicine
2014年
3期
60-62
,共3页
李坚%易延逵%蔡光先%杨永华
李堅%易延逵%蔡光先%楊永華
리견%역연규%채광선%양영화
高效液相色谱法%神安颗粒%天麻素%多指标质量控制体系
高效液相色譜法%神安顆粒%天痳素%多指標質量控製體繫
고효액상색보법%신안과립%천마소%다지표질량공제체계
HPLC method%Shenan granules%Gastrodin%multitude index quality control system
目的:建立以高效液相色谱法测定神安颗粒中天麻素含量的方法。方法:色谱柱为Kromasil C18柱,流动相为乙腈-0.05%磷酸溶液(0.5∶99.5),检测波长为220nm ,流速为1.0mL /min ,柱温为室温,进样量为10μL。结果:天麻素进样量在0.0518~0.5180μg范围内与峰面积积分值线性关系良好(r=0.9993),平均回收率为98.05%(RSD=2.92%)。结论:本方法简便、快捷、专属性强、重现性好,可用于神安颗粒苷类成分的质量控制。
目的:建立以高效液相色譜法測定神安顆粒中天痳素含量的方法。方法:色譜柱為Kromasil C18柱,流動相為乙腈-0.05%燐痠溶液(0.5∶99.5),檢測波長為220nm ,流速為1.0mL /min ,柱溫為室溫,進樣量為10μL。結果:天痳素進樣量在0.0518~0.5180μg範圍內與峰麵積積分值線性關繫良好(r=0.9993),平均迴收率為98.05%(RSD=2.92%)。結論:本方法簡便、快捷、專屬性彊、重現性好,可用于神安顆粒苷類成分的質量控製。
목적:건립이고효액상색보법측정신안과립중천마소함량적방법。방법:색보주위Kromasil C18주,류동상위을정-0.05%린산용액(0.5∶99.5),검측파장위220nm ,류속위1.0mL /min ,주온위실온,진양량위10μL。결과:천마소진양량재0.0518~0.5180μg범위내여봉면적적분치선성관계량호(r=0.9993),평균회수솔위98.05%(RSD=2.92%)。결론:본방법간편、쾌첩、전속성강、중현성호,가용우신안과립감류성분적질량공제。
Objective:To establish HPLC method for determination of Gastrodin in Shenan granules .Methods:HPLC was performed on Kromasil C18 column,the mobile phase consisted of acetonitrile -0.05% phosphoric acid solution (0.5∶99.5),the detection wave-length is 220 nm, flow rate 1.0mL /min, column temperature room temperature , sample size 10μL.Results:Gastrodin is linear with the peak area in the range of0.0518~0.5180μg (r=0.9993), the average recovery was 98.05%(RSD=2.92%).Conclusion:The established method is simple , shortcut, well specificity, reproducible, thus can be used for the quality control of Glycoside constituent quality controlling of Shenan granules .