江西中医药大学学报
江西中醫藥大學學報
강서중의약대학학보
Journal of Jiangxi University of Traditional Chinese Medicine
2014年
3期
32-34
,共3页
IL-2%NF-κB%湿热病%动物模型
IL-2%NF-κB%濕熱病%動物模型
IL-2%NF-κB%습열병%동물모형
IL-2%NF-κB%Heat Disease%Animal Model
目的：分别用内毒素和（或）肥甘饮食的方法建立大鼠湿热模型，并检测模型体内IL-2、NF-κB的表达水平，以比较不同方法造模的效果差别，并探讨大鼠湿热模型成模的机制及改进手段。方法：80只大鼠随机分为4组，第1组为空白对照，其余3组分别以肥甘饮食、内毒素、肥甘饮食＋内毒素的方法建立湿热模型，造模时间6周。用ELISA的方法检测各组大鼠IL-2、NF-κB的表达水平。结果：1．正常大鼠IL-2、NF-κB均为低表达。2．肥甘饮食组、内毒素组大鼠IL-2、NF-κB表达与正常大鼠无明显差别，肥甘饮食＋内毒素组大鼠IL-2、NF-κB表达较正常大鼠明显升高。结论：肥甘饮食＋内毒素方法对建立大鼠湿热模型效果明显，其成模的机制可能为在高血脂的基础上启动了非特异性的炎症反应。
目的：分彆用內毒素和（或）肥甘飲食的方法建立大鼠濕熱模型，併檢測模型體內IL-2、NF-κB的錶達水平，以比較不同方法造模的效果差彆，併探討大鼠濕熱模型成模的機製及改進手段。方法：80隻大鼠隨機分為4組，第1組為空白對照，其餘3組分彆以肥甘飲食、內毒素、肥甘飲食＋內毒素的方法建立濕熱模型，造模時間6週。用ELISA的方法檢測各組大鼠IL-2、NF-κB的錶達水平。結果：1．正常大鼠IL-2、NF-κB均為低錶達。2．肥甘飲食組、內毒素組大鼠IL-2、NF-κB錶達與正常大鼠無明顯差彆，肥甘飲食＋內毒素組大鼠IL-2、NF-κB錶達較正常大鼠明顯升高。結論：肥甘飲食＋內毒素方法對建立大鼠濕熱模型效果明顯，其成模的機製可能為在高血脂的基礎上啟動瞭非特異性的炎癥反應。
목적：분별용내독소화（혹）비감음식적방법건립대서습열모형，병검측모형체내IL-2、NF-κB적표체수평，이비교불동방법조모적효과차별，병탐토대서습열모형성모적궤제급개진수단。방법：80지대서수궤분위4조，제1조위공백대조，기여3조분별이비감음식、내독소、비감음식＋내독소적방법건립습열모형，조모시간6주。용ELISA적방법검측각조대서IL-2、NF-κB적표체수평。결과：1．정상대서IL-2、NF-κB균위저표체。2．비감음식조、내독소조대서IL-2、NF-κB표체여정상대서무명현차별，비감음식＋내독소조대서IL-2、NF-κB표체교정상대서명현승고。결론：비감음식＋내독소방법대건립대서습열모형효과명현，기성모적궤제가능위재고혈지적기출상계동료비특이성적염증반응。
Objective:To establish heat disease model in rats by endotoxin and (or) fatness diet respectively, and to detect IL-2, NF-κB expression in vivo of the models to compare the differences of effects by different methods of modeling , and to explore the mecha-nism of heat disease in rat model and improve methods .Methods:80 rats were randomly divided into four groups , group1 was the con-trol, and the other three groups were feeding with fatness , injecting endotoxin , both injecting endotoxin and feeding with fatness diet to create heat disease model respectively , modeling for six weeks .IL-2 and NF-κB expression in rats were detected by ELISA method . Results:1, IL-2 and NF-κB expression are low in normal rats .2, There was no significant difference of IL -2 and NF-κB expres-sion in rats among the fatness diet group , the endotoxin group and the normal group .IL-2 and NF-κB expression in group of both fatness diet +LPS were significantly higher than normal rats .Conclusions:Effect of fatness diet +endotoxin methods to establish heat disease model in rats is obvious , and the mechanism may be the initiation of non -specific inflammation on the basis high cholesterol .
