CAJ | 학술논문

目的：寻找一种快速准确、简便易行的鉴别湖北钉螺死活的方法。方法分别用0.05%中性红水溶性染液、0.5%甲基蓝染液、红墨水、伊红亚甲基蓝（MEB）染液、0.4%台盼蓝对活、死钉螺进行染色，染色30 min后压碎螺壳，体视显微镜下观察软体组织着色情况。结果0.05%中性红可将活钉螺均染成红色，死钉螺均未着色；0.5%甲基蓝染色后，活、死钉螺均不着色；红墨水可将活、死钉螺均染成红色；MEB染液可使部分活、死钉螺着色；0.4%台盼蓝可将活钉螺和部分死钉螺均染成蓝色。结论使用0.05%中性红水溶性染色液对钉螺进行染色处理，可快速、准确、客观地鉴别钉螺死活。
목적：심조일충쾌속준학、간편역행적감별호북정라사활적방법。방법분별용0.05%중성홍수용성염액、0.5%갑기람염액、홍묵수、이홍아갑기람（MEB）염액、0.4%태반람대활、사정라진행염색，염색30 min후압쇄라각，체시현미경하관찰연체조직착색정황。결과0.05%중성홍가장활정라균염성홍색，사정라균미착색；0.5%갑기람염색후，활、사정라균불착색；홍묵수가장활、사정라균염성홍색；MEB염액가사부분활、사정라착색；0.4%태반람가장활정라화부분사정라균염성람색。결론사용0.05%중성홍수용성염색액대정라진행염색처리，가쾌속、준학、객관지감별정라사활。
Objective To find out a quick,simple and convenient method of determining the viability of Oncomelania hupen-sis. Methods O. hupensis snails were stained for 30 minutes by 0.05%water soluble dye neutral red,0.5%methylene blue,red ink,methylene blue-eosin-borax(MEB)and 0.4%trypan blue,respectively. The soft tissue samples of the snails were observed by a stereoscopic microscope after crushing their shells. Results The vital snails were stained and the dead were unstained in the neutral red. The vital and dead snails were unstained in methylene blue. However,the vital and dead snails were stained in red ink. The partial vital and dead snails were stained in MEB. The vital snails were stained and the partial dead were stained in trypan blue. Conclusion The use of 0.05%water soluble dye neutral red is simple,rapid and accurate in determination of the viability of O. hupensis.