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中国血吸虫病防治杂志 중국혈흡충병방치잡지
CHINESE JOURNAL OF SCHISTOSOMIASIS CONTROL
2014年 4期 449-452 ,共4页

梁伟涛%刘华%邓瑶樑偉濤%劉華%鄧瑤

량위도%류화%산요

医学寄生虫学%多位点序列分型%管家基因%群体遗传结构分析%隐孢子虫%微孢子虫醫學寄生蟲學%多位點序列分型%管傢基因%群體遺傳結構分析%隱孢子蟲%微孢子蟲
의학기생충학%다위점서렬분형%관가기인%군체유전결구분석%은포자충%미포자충
Parasitology%Multilocus sequence typing%House-keeping gene%Population genetic structure analysis%Crypto-sporidium%Microsporidia

多位点序列分型技术（Multilocus sequence typing，MLST）具有分辨率、敏感性及特异性高等优点。在寄生虫学研究中，MLST通过扩增多个管家基因进行测序分析和对病原体进行群体遗传结构分析，可为研究虫种进化等提供更多的理论依据。本文就MLST技术的原理、方法和结果分析及其在几种寄生虫遗传结构分析方面的应用进行综述。
다위점서렬분형기술（Multilocus sequence typing，MLST）구유분변솔、민감성급특이성고등우점。재기생충학연구중，MLST통과확증다개관가기인진행측서분석화대병원체진행군체유전결구분석，가위연구충충진화등제공경다적이론의거。본문취MLST기술적원리、방법화결과분석급기재궤충기생충유전결구분석방면적응용진행종술。
Multilocus sequence typing MLST with high solution sensitivity and specificity is widely used to study the population genetic structure of pathogen by amplification and sequencing of the housekeeping genes. MLST also provides more evidence and plays an important role in parasite research. This paper reviews the principle and method of MLST and its applica-tion on population genetic structure analysis of parasites.