CAJ | 학술논문

以灯盏细辛（Erigeron breiscapus）叶片为外植体，研究不同培养基配方对灯盏细辛离体培养过程中愈伤组织、不定芽形成，壮苗培养和离体植株生根等方面的影响。研究结果表明，低浓度的细胞分裂素与生长素配比可诱导灯盏细辛愈伤组织产生，其中MS＋BA1．00mg/L＋NAA 0．50mg/L培养基配方，愈伤组织诱导率达到99．22％；不定芽诱导需要较高浓度的细胞分裂素，适宜培养基MS＋KT4．00mg/L＋IBA0．50mg/L的不定芽诱导率为38．51％，诱导系数为0．49；MS＋BA0．50mg/L＋NAA0．50 mg/L＋水解酪蛋白1000．00mg/L＋PVP1000．00 mg/L＋GA 0．50mg/L培养基可促进不定芽分化生长，形成离体植株；离体植株生根需要高浓度生长素，适宜培养基为1/2MS＋BA 0．50mg/L＋ NAA 3．00 mg/L＋IBA 3．00 mg/L＋活性炭0．30％。
이등잔세신（Erigeron breiscapus）협편위외식체，연구불동배양기배방대등잔세신리체배양과정중유상조직、불정아형성，장묘배양화리체식주생근등방면적영향。연구결과표명，저농도적세포분렬소여생장소배비가유도등잔세신유상조직산생，기중MS＋BA1．00mg/L＋NAA 0．50mg/L배양기배방，유상조직유도솔체도99．22％；불정아유도수요교고농도적세포분렬소，괄의배양기MS＋KT4．00mg/L＋IBA0．50mg/L적불정아유도솔위38．51％，유도계수위0．49；MS＋BA0．50mg/L＋NAA0．50 mg/L＋수해락단백1000．00mg/L＋PVP1000．00 mg/L＋GA 0．50mg/L배양기가촉진불정아분화생장，형성리체식주；리체식주생근수요고농도생장소，괄의배양기위1/2MS＋BA 0．50mg/L＋ NAA 3．00 mg/L＋IBA 3．00 mg/L＋활성탄0．30％。
s:Effect of different mediums on callus induction ,adventitious bud induction ,bud culture and root formation of Erigeronbreiscapus was studied ,using leaves as explants .The callus was induced from the explant of leave with mediums containing low concentration of cytokinin and auxins .The highest in-duction rate of callus reached 99 .22% on medium :MS + BA 1 .00mg/L + NAA 0 .50 mg/L .High con-centration of cytokinin was necessary to adventitious bud induction .The induction rate of adventitious bud was 38 .51% and number of shoots per explant was 0 .49 on medium :MS + KT 4 .00mg/L + IBA 0 .50mg/L .The bud easily developed into plantlet cultured on medium :MS + BA 0 .50mg/L + NAA 0 .50 mg/L+casein hydrolysate 1000 .00 mg/L +PVP 1000 .00 mg/L+ GA 0 .50mg/L .Root formation need medium containing high concentration of auxins .The optimal medium for root formation was 1/2MS+ BA 0 .50mg/L + NAA 3 .00 mg/L + IBA 3 .00 mg/L + activated charcole 0 .30% .