CAJ | 학술논문

建立了高效液相色谱法（ HPLC ）测定复方陆朴微乳剂中厚朴酚与和厚朴酚含量的方法。样品经85℃水浴去除溶剂后，加入甲醇溶解超声处理30 min、过滤，取滤液，测定其中厚朴酚与和厚朴酚的含量。色谱条件：采用 ZORBAX SB-C18色谱柱（250 mm ×4?6 mm，5μm），流动相为0?5％磷酸-甲醇、流速为0?70 mL/min、检测波长为294 nm、柱温30℃，进样量10μL。结果表明：厚朴酚在0?074～0?518μg/mL（r＝0?9997）、和厚朴酚在0?152～1?064μg/mL（r＝0?9998）范围内，其质量浓度和峰面积之间均呈良好的线性关系；厚朴酚的添加回收率在98?8％～100?2％之间，相对标准偏差（RSD）为0?70％～1?0％（n＝6）；和厚朴酚的添加回收率在98?5％～99?5％之间， RSD为0?90％～1?1％（ n＝6），5个不同批次的复方陆朴微乳剂中厚朴酚、和厚朴酚的平均含量分别为2?6924和1?2165 mg/mL。该方法操作简便、准确度高、重现性好，可作为复方陆朴微乳剂中厚朴酚与和厚朴酚含量的测定方法。
건립료고효액상색보법（ HPLC ）측정복방륙박미유제중후박분여화후박분함량적방법。양품경85℃수욕거제용제후，가입갑순용해초성처리30 min、과려，취려액，측정기중후박분여화후박분적함량。색보조건：채용 ZORBAX SB-C18색보주（250 mm ×4?6 mm，5μm），류동상위0?5％린산-갑순、류속위0?70 mL/min、검측파장위294 nm、주온30℃，진양량10μL。결과표명：후박분재0?074～0?518μg/mL（r＝0?9997）、화후박분재0?152～1?064μg/mL（r＝0?9998）범위내，기질량농도화봉면적지간균정량호적선성관계；후박분적첨가회수솔재98?8％～100?2％지간，상대표준편차（RSD）위0?70％～1?0％（n＝6）；화후박분적첨가회수솔재98?5％～99?5％지간， RSD위0?90％～1?1％（ n＝6），5개불동비차적복방륙박미유제중후박분、화후박분적평균함량분별위2?6924화1?2165 mg/mL。해방법조작간편、준학도고、중현성호，가작위복방륙박미유제중후박분여화후박분함량적측정방법。
A high performance liquid chromatography ( HPLC ) method was developed for determinating contents of magnolol and honokiol in Lupo compound microemulsion formulation. Samples were extracted with methanol for 30 minutes after them were evaporated by solvent completely at temperature of 85 ℃, obtaining the filtering filtrate for determination. The chromatographic conditions were as follow: ZORBAX SB-C18 (250 mm × 4?6 mm, 5 μm) was used with the mobile phase of 0?5% phosphoric acid-methanol and the flow rate was 0?70 mL/min, at the detection wavelength of 294 nm, and the column temperature was 30 ℃. The results showed that the linear relationship of the magnolol density and peak areas was in the range of 0?074 -0?518 μg/mL ( r=0?999 7 ) , and that of the honokiol was in the range of 0?152 -1?064 μg/mL ( r=0?999 8 ) . The average recovery of magnolol was from 98?8% to 100?2% with RSD from 0?70% to 1?0% ( n=6 ) , and that of honokiol was from 98?5% to 99?5% with RSD from 0?90% to 1?1% ( n=6 ) . In five different batches of Lupo compound microemulsion formulation, the average content of magnolol was 2?692 4 mg/mL and that of honokiol was 1?216 5 mg/mL. The method is simple,quick, accurate and reproducible and can be used to determinate the magnolol and honokiol contents in Lupo compound microemulsion formulation.