CAJ | 학술논문

目的：观察远志总皂苷（TEN）对阿尔茨海默病（AD）模型大鼠海马CA1区神经颗粒素（Ng）以及磷酸化的钙/钙调蛋白依赖性蛋白激酶Ⅱα亚基（ pCaMKⅡα）表达的影响，探讨TEN防治AD的作用机制。方法将60只雄性Wistar大鼠随机分为对照组，模型组，TEN低剂量治疗组（12．5mg/ml）和TEN高剂量治疗组（37．5 mg/ml），每组15只，模型组予以腹腔注射 D-半乳糖（D-gal）致衰并联合鹅膏覃氨酸（IBO）定向Meynert基底核损毁法建立AD大鼠模型，TEN低、高剂量治疗组则在造模的同时TEN灌胃治疗8w，对照组则用等体积的生理盐水代替D-gal和IBO。采用免疫组化方法来检测大鼠海马CA1区Ng及pCaMKⅡα的表达。结果模型组大鼠海马CA1区Ng 及pCaMKⅡα平均吸光度值比对照组显著降低（ P＜0．01）；TEN治疗组两组平均吸光度值均较模型组显著增高（ P＜0．01）；且TEN高剂量治疗组（37．5 mg/ml）的平均吸光度值比TEN低剂量治疗组（12．5mg/ml）显著增高（P＜0．01）。结论 TEN可显著升高AD模型大鼠海马CA1区Ng 及pCaMKⅡα的表达，且具有剂量依赖性。
목적：관찰원지총조감（TEN）대아이자해묵병（AD）모형대서해마CA1구신경과립소（Ng）이급린산화적개/개조단백의뢰성단백격매Ⅱα아기（ pCaMKⅡα）표체적영향，탐토TEN방치AD적작용궤제。방법장60지웅성Wistar대서수궤분위대조조，모형조，TEN저제량치료조（12．5mg/ml）화TEN고제량치료조（37．5 mg/ml），매조15지，모형조여이복강주사 D-반유당（D-gal）치쇠병연합아고담안산（IBO）정향Meynert기저핵손훼법건립AD대서모형，TEN저、고제량치료조칙재조모적동시TEN관위치료8w，대조조칙용등체적적생리염수대체D-gal화IBO。채용면역조화방법래검측대서해마CA1구Ng급pCaMKⅡα적표체。결과모형조대서해마CA1구Ng 급pCaMKⅡα평균흡광도치비대조조현저강저（ P＜0．01）；TEN치료조량조평균흡광도치균교모형조현저증고（ P＜0．01）；차TEN고제량치료조（37．5 mg/ml）적평균흡광도치비TEN저제량치료조（12．5mg/ml）현저증고（P＜0．01）。결론 TEN가현저승고AD모형대서해마CA1구Ng 급pCaMKⅡα적표체，차구유제량의뢰성。
Objective To observe the effects of tenuigenin ( TEN) on expression of neurogranin ( Ng) and the phosphorylation of calcium/calmodulin-dependent protein kinasetype Ⅱ-alpha ( pCaMKⅡα) in Alzheimer ’ s disease model rats and its possible mechanism .Methods 60 male Wistar rats were divided randomly into four groups:control group,model group,low-dose TEN treatment group(12.5mg/ml)and high-dose TEN treatment group (37.5 mg/ml),each with 15 rats.Model group,low-dose(12.5mg/ml)and high-dose TEN treatment group (37.5 mg/ml)were made by injecting ibotenic acid into Meynertbasal nuclei of aging rat induced by D -gal.At the same time, low-dose TEN treatment group and high-dose TEN treatment group were respectively administrated by gavage for 8 weeks with 12.5mg/ml and 37.5 mg/ml TEN, and the control group was injected with equal volume of normal saline solution instead of D-gal and IBO .The expressions of Ng and pCaMKⅡαin the hippocampus CA 1 area were measured by immunohistochemistry method.Results Compared with the control group , the average absorbance values of Ng and pCaMKⅡαin the model group were decreased significantly ( P<0.01 ) .Compared with the model group , the average absorbance values of Ng and pCaMKⅡαin low-dose TEN treatment group and high-dose TEN treatment group increased significantly ( P<0.01 ) .The average absorbance values of Ng and pCaMKⅡαin high-dose TEN treatment group(37.5 mg/ml) increased significantly than in low-dose TEN treatment group(12.5mg/ml)(P<0.01). Conclusion TEN can dose-dependently increase the expression of Ng and pCaMKⅡαin the hippocampus CA 1 area of Alzheimer’s disease model rats.