脑与神经疾病杂志
腦與神經疾病雜誌
뇌여신경질병잡지
JOURNAL OF BRAIN AND NERVOUS DISEASES
2014年
4期
241-244
,共4页
李彩格%唐蔚东%张松筠%张力辉%王瑞英%王绵
李綵格%唐蔚東%張鬆筠%張力輝%王瑞英%王綿
리채격%당위동%장송균%장력휘%왕서영%왕면
糖尿病%穹窿下器%核转录因子-κB%Wistar大鼠
糖尿病%穹窿下器%覈轉錄因子-κB%Wistar大鼠
당뇨병%궁륭하기%핵전록인자-κB%Wistar대서
Diabetes%Subfornical organ%Nuclear transcription factor kappa B%Wistar rats
目的:观察核转录因子-κB ( NF-κB)在不同时期糖尿病大鼠穹窿下器( SFO)中的表达并探讨其表达的意义。方法雄性Wistar大鼠,模型组每只大鼠给予链脲佐菌素60mg· kg-1,腹腔一次性注射,建立1型糖尿病大鼠模型。用免疫组织化学染色方法观察NF-κB在正常大鼠及2、4、8 w糖尿病大鼠SFO细胞内的表达。结果对照组NF-κB免疫阳性细胞稀少,胞核呈浅棕黄色,平均吸光度为156.42±7.25。2w组NF-κB免疫强阳性细胞SFO内均匀分布,核呈棕褐色,高度表达,为强阳性,平均吸光度为183.72±8.35。4w组SFO NF-κB的表达水平有所下降但仍高于正常对照组,平均吸光度为169.55±5.84。8w组NF-κB免疫强阳性细胞的表达与正常对照组无明显区别,平均吸光度为158.32±8.72。平均吸光度测定:2w组>4w组>8w组, P ﹤0.05,8w组与对照组比较P﹥0.05。结论 NF-κB在糖尿病大鼠SFO细胞内呈短暂一过性的表达增强,NF-κB途经可能在糖尿病大鼠 SFO细胞损伤中起着重要的作用。
目的:觀察覈轉錄因子-κB ( NF-κB)在不同時期糖尿病大鼠穹窿下器( SFO)中的錶達併探討其錶達的意義。方法雄性Wistar大鼠,模型組每隻大鼠給予鏈脲佐菌素60mg· kg-1,腹腔一次性註射,建立1型糖尿病大鼠模型。用免疫組織化學染色方法觀察NF-κB在正常大鼠及2、4、8 w糖尿病大鼠SFO細胞內的錶達。結果對照組NF-κB免疫暘性細胞稀少,胞覈呈淺棕黃色,平均吸光度為156.42±7.25。2w組NF-κB免疫彊暘性細胞SFO內均勻分佈,覈呈棕褐色,高度錶達,為彊暘性,平均吸光度為183.72±8.35。4w組SFO NF-κB的錶達水平有所下降但仍高于正常對照組,平均吸光度為169.55±5.84。8w組NF-κB免疫彊暘性細胞的錶達與正常對照組無明顯區彆,平均吸光度為158.32±8.72。平均吸光度測定:2w組>4w組>8w組, P ﹤0.05,8w組與對照組比較P﹥0.05。結論 NF-κB在糖尿病大鼠SFO細胞內呈短暫一過性的錶達增彊,NF-κB途經可能在糖尿病大鼠 SFO細胞損傷中起著重要的作用。
목적:관찰핵전록인자-κB ( NF-κB)재불동시기당뇨병대서궁륭하기( SFO)중적표체병탐토기표체적의의。방법웅성Wistar대서,모형조매지대서급여련뇨좌균소60mg· kg-1,복강일차성주사,건립1형당뇨병대서모형。용면역조직화학염색방법관찰NF-κB재정상대서급2、4、8 w당뇨병대서SFO세포내적표체。결과대조조NF-κB면역양성세포희소,포핵정천종황색,평균흡광도위156.42±7.25。2w조NF-κB면역강양성세포SFO내균균분포,핵정종갈색,고도표체,위강양성,평균흡광도위183.72±8.35。4w조SFO NF-κB적표체수평유소하강단잉고우정상대조조,평균흡광도위169.55±5.84。8w조NF-κB면역강양성세포적표체여정상대조조무명현구별,평균흡광도위158.32±8.72。평균흡광도측정:2w조>4w조>8w조, P ﹤0.05,8w조여대조조비교P﹥0.05。결론 NF-κB재당뇨병대서SFO세포내정단잠일과성적표체증강,NF-κB도경가능재당뇨병대서 SFO세포손상중기착중요적작용。
Objective To observe the expression of changes of nuclear transcription factor kappa B ( NF-κB) in subfornical organ ( SFO) in different stages of diabetic rat .Methods Male Wistar rats , each rat of model group was given streptozotocin ( STZ) 60mg · kg-1 by intraperitoneal injection all at once to establish type 1 diabetic rat model .Immunohistochemical staining was used to observe the expression of NF-κB in subfornical organ of normal rats and diabetic rats in 2,4,8,12 weeks.Results Numbers of immunopositive cells of NF-κB were scarce, nuclei were light brown yellow,and the average optical density was 156.42±7.25.2 weeks group, NF-κB-positive cells in SFO were evenly distributed , highly expressed, nuclei were brown, and the average optical density was 183.72±8.35.4 weeks group , the expression level of NF-κB positive cells in SFO was decreased but still higher than the normal control group, the average optical density was .There was no significant difference between 8 weeks group and normal control group in the expression of NF-κB strongly positive cells , the average optical density was 158.32 ±8.72. Average optical density measurement results:2 weeks group>4 weeks>8 weeks group , with statistical significance (P<0.05), 8 weeks group and the control group had no significant difference ( P>0.05).Conclusion Among different diabetic rat models of different times , NF-κB expression in the SFO is transient enhanced , which indicates that NF-κB pathway may play an important role in damage of SFO in diabetic rats .