CAJ | 학술논문

目的：评定采用高效液相色谱(HPLC)法测定兔眼玻璃体中丝裂霉素C（mitomycin C，MMC）浓度的不确定度。方法：对HPLC法测定兔眼玻璃体中MMC浓度的全过程进行分析，对于分析过程中称量、溶液配制、分析仪器以及标准曲线拟和等影响因素引起的不确定度进行了逐层分析，用A类评定程序评价了分析过程中随机效应引起的不确定度，用B类评定程序评价了分析过程的其他因素引起的不确定度，最后根据各分量计算出合成不确定度并进行了扩展。结果：置信概率P为95%时，玻璃体低(0.1μg·mL-1)、中(0.8μg·mL-1)、高(2.5μg·mL-1)浓度MMC的扩展不确定度分别为0.003μg·mL-1、0.02μg·mL-1、0.06μg·mL-1。结论：本方法适用于HPLC法测定兔眼玻璃体中MMC浓度的不确定度评定,该方法的确立对于复杂生物基质分析过程的不确定度研究具有重要意义。
목적：평정채용고효액상색보(HPLC)법측정토안파리체중사렬매소C（mitomycin C，MMC）농도적불학정도。방법：대HPLC법측정토안파리체중MMC농도적전과정진행분석，대우분석과정중칭량、용액배제、분석의기이급표준곡선의화등영향인소인기적불학정도진행료축층분석，용A류평정정서평개료분석과정중수궤효응인기적불학정도，용B류평정정서평개료분석과정적기타인소인기적불학정도，최후근거각분량계산출합성불학정도병진행료확전。결과：치신개솔P위95%시，파리체저(0.1μg·mL-1)、중(0.8μg·mL-1)、고(2.5μg·mL-1)농도MMC적확전불학정도분별위0.003μg·mL-1、0.02μg·mL-1、0.06μg·mL-1。결론：본방법괄용우HPLC법측정토안파리체중MMC농도적불학정도평정,해방법적학립대우복잡생물기질분석과정적불학정도연구구유중요의의。
Objective:To evaluate the uncertainty of MMC in rabbit vitreous by HPLC. Method:The uncertainty caused by vari-ous factors in the whole process of determination, including balancing, solution preparation, calibration fitting and the equipment, was estimated. The uncertainty caused by random effects was evaluated with type A and others were by type B. The combined uncertainty was calculated with all the components. Results:The expanded uncertainty for low, medium and high level of MMC was 0.003, 0.02 and 0.06μg·mL-1 , respectively ( P=95%). Conclusion:This method is applicable for the uncertainty evaluation of MMC determina-tion in rabbit vitreous. It provides an important reference for the uncertainty evaluation for the determination in complex bio-matrix.