CAJ | 학술논문

目的：比较分析正常生育男性与精索静脉曲张（varicocele, VC）不育患者差异表达的精子蛋白质。方法应用计算机辅助精子分析系统行精液分析，采取Percoll密度梯度离心法提取正常生育男性与VC不育患者精子，利用双向电泳（two-dimensional electrophoresis,2-DE）分离精子蛋白质，建立并优化精子蛋白质2-DE图谱，利用PDQuest8.0软件分析两组蛋白质的差异表达。结果正常生育组精子活力高于VC不育组，两组比较差异有统计学意义（P＜0.05），两组精子密度比较差异无统计学意义（P＞0.05）。获得了分辨率和重复性均较好的2-DE图谱，两组差异点共36个，VC不育组有11个蛋白位点表达上调，19个蛋白位点表达下调，2个在VC不育组特异性表达，4个在正常生育组特异性表达。结论 VC不育患者与正常生育男性的精子蛋白存在差异，这些差异蛋白质的分离为深入探讨VC导致不育的分子机制奠定了基础。
목적：비교분석정상생육남성여정색정맥곡장（varicocele, VC）불육환자차이표체적정자단백질。방법응용계산궤보조정자분석계통행정액분석，채취Percoll밀도제도리심법제취정상생육남성여VC불육환자정자，이용쌍향전영（two-dimensional electrophoresis,2-DE）분리정자단백질，건립병우화정자단백질2-DE도보，이용PDQuest8.0연건분석량조단백질적차이표체。결과정상생육조정자활력고우VC불육조，량조비교차이유통계학의의（P＜0.05），량조정자밀도비교차이무통계학의의（P＞0.05）。획득료분변솔화중복성균교호적2-DE도보，량조차이점공36개，VC불육조유11개단백위점표체상조，19개단백위점표체하조，2개재VC불육조특이성표체，4개재정상생육조특이성표체。결론 VC불육환자여정상생육남성적정자단백존재차이，저사차이단백질적분리위심입탐토VC도치불육적분자궤제전정료기출。
Objective To study the differential expression of sperm proteins between fertile men and infertile men with varicocele(VC). Methods Sperm samples were analyzed by computer-assisted semen analysis system. Sperm proteins were extracted by percoll density gradient centrifugation, and the separation of total proteins was performed by two-dimensional electrophoresis(2-DE). The differentially expressed proteins of two groups were analyzed with PDQuest software. Results The sperm motility in infertile men with VC demonstrated a significant decrease compared with the fertile men(P<0.05), and there was no significant difference between two groups in sperm concentration(P>0.05). Protein profile of sperm was acquired with clear background, high resolution and better reproduction.Total of 36 different proteins were found, including 11 upregulated protein and 19 downregulated proteins in infertile men with VC group, and two proteins only expressed in infertile men with VC group, the other four proteins only expressed in fertile men group. Conclusion There are differential expression of sperm proteins between fertile men and infertile men with VC. Differential proteins analysis will be helpful to explore the molecular mechanism of VC-associated male infertility.