生态环境学报
生態環境學報
생태배경학보
ECOLOGY AND ENVIRONMENT
2014年
5期
884-889
,共6页
镉胁迫%玉米%蛋白%酶系统
鎘脅迫%玉米%蛋白%酶繫統
력협박%옥미%단백%매계통
cadmium%corn CT38%protein%enzyme system
玉米（Zea mays L.）被认为是镉吸收强、生物量大的经济作物。采用盆栽实验研究镉胁迫对玉米CT38植株生长及抗氧化酶活性的影响。通过向土壤中添加不同浓度的镉，设定1、5、15、50、100 mg·kg-1共5个镉浓度，研究不同镉浓度长期处理下对玉米CT38生长发育的影响。结果表明：处理90 d时玉米株高在镉处理浓度15、50、100 mg·kg-1分别比对照下降了18.2%、56.1%、59.8%，鲜质量分别比对照下降了53.3%、66.9%、77.9%。镉胁迫下玉米植株叶中抗氧化酶系统的POD、CAT和APX活性变化规律存在共同点，随镉处理浓度升高，基本呈单峰曲线变化，SOD活性变化情况较复杂。处理90 d时，镉处理浓度为1、5、15、50、100 mg·kg-1时，玉米CT38叶片POD活性分别比同期对照下降了12.1%、49.8%、60%、64.9%、75.1%；APX活性分别下降了54.4%、58.9%、65.6%、79.5%、85.3%；CAT活性分别增加了12.5%、18.8%、31.3%、65.6%、143.8%。CAT活性在玉米生长发育阶段随镉浓度升高呈现增加趋势镉使玉米叶片内酶含量上升，可溶性蛋白总量增加，为玉米对抗镉胁迫提供生理基础，对抗镉胁迫影响，提高POD活性，加速玉米植株内过氧化物清除速度。在相对低的镉浓度作用下，玉米叶片CAT活性升高，抵抗低浓度镉胁迫条件下产生的过氧化氢，从而适应逆境环境。
玉米（Zea mays L.）被認為是鎘吸收彊、生物量大的經濟作物。採用盆栽實驗研究鎘脅迫對玉米CT38植株生長及抗氧化酶活性的影響。通過嚮土壤中添加不同濃度的鎘，設定1、5、15、50、100 mg·kg-1共5箇鎘濃度，研究不同鎘濃度長期處理下對玉米CT38生長髮育的影響。結果錶明：處理90 d時玉米株高在鎘處理濃度15、50、100 mg·kg-1分彆比對照下降瞭18.2%、56.1%、59.8%，鮮質量分彆比對照下降瞭53.3%、66.9%、77.9%。鎘脅迫下玉米植株葉中抗氧化酶繫統的POD、CAT和APX活性變化規律存在共同點，隨鎘處理濃度升高，基本呈單峰麯線變化，SOD活性變化情況較複雜。處理90 d時，鎘處理濃度為1、5、15、50、100 mg·kg-1時，玉米CT38葉片POD活性分彆比同期對照下降瞭12.1%、49.8%、60%、64.9%、75.1%；APX活性分彆下降瞭54.4%、58.9%、65.6%、79.5%、85.3%；CAT活性分彆增加瞭12.5%、18.8%、31.3%、65.6%、143.8%。CAT活性在玉米生長髮育階段隨鎘濃度升高呈現增加趨勢鎘使玉米葉片內酶含量上升，可溶性蛋白總量增加，為玉米對抗鎘脅迫提供生理基礎，對抗鎘脅迫影響，提高POD活性，加速玉米植株內過氧化物清除速度。在相對低的鎘濃度作用下，玉米葉片CAT活性升高，牴抗低濃度鎘脅迫條件下產生的過氧化氫，從而適應逆境環境。
옥미（Zea mays L.）피인위시력흡수강、생물량대적경제작물。채용분재실험연구력협박대옥미CT38식주생장급항양화매활성적영향。통과향토양중첨가불동농도적력，설정1、5、15、50、100 mg·kg-1공5개력농도，연구불동력농도장기처리하대옥미CT38생장발육적영향。결과표명：처리90 d시옥미주고재력처리농도15、50、100 mg·kg-1분별비대조하강료18.2%、56.1%、59.8%，선질량분별비대조하강료53.3%、66.9%、77.9%。력협박하옥미식주협중항양화매계통적POD、CAT화APX활성변화규률존재공동점，수력처리농도승고，기본정단봉곡선변화，SOD활성변화정황교복잡。처리90 d시，력처리농도위1、5、15、50、100 mg·kg-1시，옥미CT38협편POD활성분별비동기대조하강료12.1%、49.8%、60%、64.9%、75.1%；APX활성분별하강료54.4%、58.9%、65.6%、79.5%、85.3%；CAT활성분별증가료12.5%、18.8%、31.3%、65.6%、143.8%。CAT활성재옥미생장발육계단수력농도승고정현증가추세력사옥미협편내매함량상승，가용성단백총량증가，위옥미대항력협박제공생리기출，대항력협박영향，제고POD활성，가속옥미식주내과양화물청제속도。재상대저적력농도작용하，옥미협편CAT활성승고，저항저농도력협박조건하산생적과양화경，종이괄응역경배경。
Maize (Zea mays L.) was considered to be the cadmium absorption strong, large biomass crops. The pot experiment wasconducted to study effects of cadmium stress on growth and antioxidant enzyme activity of maize CT38 plant. By adding different concentrations of cadmium in the soil, set 1, 5, 15, 50, 100 mg·kg-1, a total of 5 Cd concentration, to study the effects of different concentration of cadmium long-term treatment on corn growth and development of CT38. The results showed that, Maize plant height in the Cd concentration 15, 50, 100 mg·kg-1 were decreased by 18.2%, 56.1%, 59.8%, fresh weight were decreased by 53.3%, 66.9%, 77.9% when dealing with 90 d. There were common points of maize leaf antioxidase system in POD, CAT, and APX activity change of Cd stress, with the Cd concentration increased, the basic was the single peak curve, the change of SOD activity was more complex. The treatment of 90 d, the Cd concentration was 1, 5, 15, 50, 100 mg·kg-1, POD activity in leaves of maize CT38 respectively over the same period decreased by 12.1%, 49.8%, 60%, 64.9%, 75.1%. APX activity were decreased by 54.4%, 58.9%, 65.6%, 79.5%, 85.3%. The activity of CAT were increased by 12.5%, 18.8%, 31.3%, 65.6%, 143.8%. The activity of CAT in maize growth stages increased cadmium content in maize leaves enzyme increased with cadmium concentration. Total soluble protein increased, provide the physiological basis for maize against cadmium stress, against the effects of cadmium stress. Increased POD activity, accelerated the corn plant peroxide scavenging rate. Cadmium concentrations in the role of relatively low, increased CAT activity of maize, resulting in resistance to low concentrations of cadmium stress conditions of hydrogen peroxide, so as to adapt to the stress environment.
