生态环境学报
生態環境學報
생태배경학보
ECOLOGY AND ENVIRONMENT
2014年
5期
870-875
,共6页
土霉素%吸附%草甸土%离子强度%离子类型
土黴素%吸附%草甸土%離子彊度%離子類型
토매소%흡부%초전토%리자강도%리자류형
oxytetracycline%sorption%meadow%cation strength%different cations
土霉素是四环素类抗生素中使用较为广泛的一种,探索土霉素在土壤中的吸附解吸规律对抗生素环境风险评价与污染控制具有重要的理论与现实意义。通过改变土壤水溶液中离子强度及类型研究其对土霉素在草甸土中吸附的影响,以期为评价该种抗生素对草甸土土壤环境风险提供科学依据。土霉素测定方法选择高效液相色谱法。以 V(乙腈)∶V[NaH2PO4(0.01 mol·L-1)]=25∶75为流动相,在流速为1 mL·min-1,检测波长为355 nm条件下进行测定。试验以OECD Guideline 106为基础,采用批平衡法研究不同离子强度(0.01、0.03、0.05、0.08、0.10 mol·L-1CaCl2溶液)和不同阳离子(0.01 mol·L-1的NaCl、KCl、MgCl2溶液)对土霉素在草甸土中的吸附影响。结果表明:随着 CaCl2浓度的增加,土霉素在草甸土中的吸附量呈降低趋势,且不同浓度的土霉素在草甸土中吸附量受离子强度的影响是不同的。土霉素浓度较低时,其在草甸土中吸附能力受离子强度影响较土霉素浓度高时小。以土壤吸附系数 Kd衡量土壤吸附抗生素能力,当土壤样品中离子强度增大时,其对应Kd值呈非线性趋势减小。这说明,在某一Ca2+浓度范围内,其对吸附的影响较其他浓度范围要大。对相邻CaCl2浓度处理得出的Kd值进行t检验,在CaCl2溶液浓度在0.01与0.03 mol·L-1间得出的Kd值存在显著性差异(p≤0.05),在0.03、0.05、0.08、0.10 mol·L-1浓度间得出的Kd值均没有显著性差异(p>0.05)。这一结果说明当CaCl2溶液浓度在0.01~0.03 mol·L-1时,其对土霉素在草甸土中的吸附影响较其他浓度范围大。以Freundlich方程对NaCl、KCl、MgCl2溶液条件下土霉素在草甸土中的吸附进行拟合,土样lg Kf值为lg Kf(Mg2+)<lg Kf(Na+)<lg Kf(K+),经过方差分析,不同阳离子对lg Kf的影响均未达到显著性差异(p>0.05)。这说明,不同阳离子对土霉素在草甸土上的吸附量(lg Kf)影响的差异并不显著。
土黴素是四環素類抗生素中使用較為廣汎的一種,探索土黴素在土壤中的吸附解吸規律對抗生素環境風險評價與汙染控製具有重要的理論與現實意義。通過改變土壤水溶液中離子彊度及類型研究其對土黴素在草甸土中吸附的影響,以期為評價該種抗生素對草甸土土壤環境風險提供科學依據。土黴素測定方法選擇高效液相色譜法。以 V(乙腈)∶V[NaH2PO4(0.01 mol·L-1)]=25∶75為流動相,在流速為1 mL·min-1,檢測波長為355 nm條件下進行測定。試驗以OECD Guideline 106為基礎,採用批平衡法研究不同離子彊度(0.01、0.03、0.05、0.08、0.10 mol·L-1CaCl2溶液)和不同暘離子(0.01 mol·L-1的NaCl、KCl、MgCl2溶液)對土黴素在草甸土中的吸附影響。結果錶明:隨著 CaCl2濃度的增加,土黴素在草甸土中的吸附量呈降低趨勢,且不同濃度的土黴素在草甸土中吸附量受離子彊度的影響是不同的。土黴素濃度較低時,其在草甸土中吸附能力受離子彊度影響較土黴素濃度高時小。以土壤吸附繫數 Kd衡量土壤吸附抗生素能力,噹土壤樣品中離子彊度增大時,其對應Kd值呈非線性趨勢減小。這說明,在某一Ca2+濃度範圍內,其對吸附的影響較其他濃度範圍要大。對相鄰CaCl2濃度處理得齣的Kd值進行t檢驗,在CaCl2溶液濃度在0.01與0.03 mol·L-1間得齣的Kd值存在顯著性差異(p≤0.05),在0.03、0.05、0.08、0.10 mol·L-1濃度間得齣的Kd值均沒有顯著性差異(p>0.05)。這一結果說明噹CaCl2溶液濃度在0.01~0.03 mol·L-1時,其對土黴素在草甸土中的吸附影響較其他濃度範圍大。以Freundlich方程對NaCl、KCl、MgCl2溶液條件下土黴素在草甸土中的吸附進行擬閤,土樣lg Kf值為lg Kf(Mg2+)<lg Kf(Na+)<lg Kf(K+),經過方差分析,不同暘離子對lg Kf的影響均未達到顯著性差異(p>0.05)。這說明,不同暘離子對土黴素在草甸土上的吸附量(lg Kf)影響的差異併不顯著。
토매소시사배소류항생소중사용교위엄범적일충,탐색토매소재토양중적흡부해흡규률대항생소배경풍험평개여오염공제구유중요적이론여현실의의。통과개변토양수용액중리자강도급류형연구기대토매소재초전토중흡부적영향,이기위평개해충항생소대초전토토양배경풍험제공과학의거。토매소측정방법선택고효액상색보법。이 V(을정)∶V[NaH2PO4(0.01 mol·L-1)]=25∶75위류동상,재류속위1 mL·min-1,검측파장위355 nm조건하진행측정。시험이OECD Guideline 106위기출,채용비평형법연구불동리자강도(0.01、0.03、0.05、0.08、0.10 mol·L-1CaCl2용액)화불동양리자(0.01 mol·L-1적NaCl、KCl、MgCl2용액)대토매소재초전토중적흡부영향。결과표명:수착 CaCl2농도적증가,토매소재초전토중적흡부량정강저추세,차불동농도적토매소재초전토중흡부량수리자강도적영향시불동적。토매소농도교저시,기재초전토중흡부능력수리자강도영향교토매소농도고시소。이토양흡부계수 Kd형량토양흡부항생소능력,당토양양품중리자강도증대시,기대응Kd치정비선성추세감소。저설명,재모일Ca2+농도범위내,기대흡부적영향교기타농도범위요대。대상린CaCl2농도처리득출적Kd치진행t검험,재CaCl2용액농도재0.01여0.03 mol·L-1간득출적Kd치존재현저성차이(p≤0.05),재0.03、0.05、0.08、0.10 mol·L-1농도간득출적Kd치균몰유현저성차이(p>0.05)。저일결과설명당CaCl2용액농도재0.01~0.03 mol·L-1시,기대토매소재초전토중적흡부영향교기타농도범위대。이Freundlich방정대NaCl、KCl、MgCl2용액조건하토매소재초전토중적흡부진행의합,토양lg Kf치위lg Kf(Mg2+)<lg Kf(Na+)<lg Kf(K+),경과방차분석,불동양리자대lg Kf적영향균미체도현저성차이(p>0.05)。저설명,불동양리자대토매소재초전토상적흡부량(lg Kf)영향적차이병불현저。
Oxytetracycline (OTC), one of the tetracycline class of antibiotics, is more widely used in China. It has important significance theoretically and practically to explore the law of adsorption-desorption of OTC in soil for environmental riskassessment and pollution control of antibiotics. In this study, ionic strength and type in soil water solution were changed to reveal the influence on the adsorption of OTC in meadow soil, aiming at providing a scientific basis for evaluating the environmental risk of OTC in meadow soil. HPLC was the determination method of OTC, which acetonitrile: NaH2PO4 (0.01 mol·L-1) =25∶75 (volume ratio) was as mobile phase at a flow rate of 1 mL·min-1and detection wavelength was 355 nm. On the basis of OECD Guideline 106, bath sorption methods were employed to reveal the effect of cation strength (ranging from 0.01 to 0.10 mol·L-1CaCl2) and different cation (0.01 mol·L-1solution of NaCl, KCl, MgCl2) on OTC sorption process in the meadow soil. Results indicated that the adsorption capacity of OTC in meadow soil gradually decreased with the increasing of concentrations of CaCl2, and the adsorption capacity of different concentrations of OTC in meadow soil was affected by different ionic strength. Compared with higher OTC concentrations, the influence of ionic strength was negligible to the sorption at lower OTC concentrations. The soil adsorption coefficient (Kd) was used to measure the ability of adsorption of OTC in meadow. When ionic strength in soil samples increased, its correspondingKdwould decreased with a non-linear trend. This result showed that there definitely existed a certain concentration range of Ca2+ that had the biggest effect on adsorption than other concentration range. TheKd of the adjacent concentration CaCl2 were handled with T-test, showing that the value ofKd existed a significant difference when the concentration ranges of CaCl2 were from 0.01 mol·L-1 to 0.03 mol·L-1(p≤0.05), while when the the concentration ranges of CaCl2were 0.03, 0.05, 0.08, 0.10 mol·L-1 (p>0.05)were not. The results suggested that when the concentrations of CaCl2 solution were 0.01-0.03 mol·L-1, its adsorption of oxytetracycline in the meadow has greater influence than other ranges of concentrations. Using Freundlich model fitted with the adsorption of oxytetracycline in meadow soils under NaCl, KCl, MgCl2 solution conditions, the lgKf value order of soil samples was lgKf (Mg2+) <lgKf(Na+) <lgKf (K+). And the effect of different cations on lgKf did not reach significant difference (p>0.05) through analysis of variance. This suggested that different cation adsorption capacity(lgKf) of oxytetracycline on the meadow soil was not significant .