CAJ | 학술논문

研究了多效唑(paclobutrazol)在番茄（Lycopersicon eseulentum）和土壤中的残留分析方法及残留动态。建立番茄和土壤样品中多效唑残留的固相萃取-高效液相色谱（SPE-HPLC）检测方法，样品用乙腈提取，再用φ(甲醇-二氯甲烷)=5∶95混合溶剂经LC-NH2固相萃取柱净化，以φ(乙腈-水)=55∶45作流动相，Shiseido C18色谱柱（4.6 mm×250 mm，5μm）于222 nm波长检测，外标法定量。在0.1~5.0 mg·L-1范围内，多效唑峰面积与其质量浓度之间呈良好线性关系，相关系数为0.9995。采用田间试验方法，在番茄幼苗期施用不同多效唑质量分数50、100、200、600 mg·kg-1，研究在不同处理时间1、6 h，1、2、3、7、14、21、30、45、60 d，多效唑在番茄以及土壤中的残留动态变化。添加质量分数水平为0.05、0.1、0.5 mg·kg-1时，多效唑在果实、植株和土壤中的添加回收率分别为92.45%~103.70%、94.52%~98.85%和94.30%~102.10%，变异系数分别为3.69%~5.00%、1.58%~4.53%和1.28%~3.35%。结果表明：多效唑在番茄植株和土壤中的降解规律均符合一级动力学方程C＝Coe-kt。当施用质量分数为600 mg·kg-1时，其在番茄植株中的残留半衰期为1.66 d，在土壤中的半衰期为2.78 d；在植株中的降解速率大于在土壤中的降解速率。按照推荐使用的施用浓度，采收时多效唑在番茄和土壤中无残留，证明推荐施用浓度是合理的。
연구료다효서(paclobutrazol)재번가（Lycopersicon eseulentum）화토양중적잔류분석방법급잔류동태。건립번가화토양양품중다효서잔류적고상췌취-고효액상색보（SPE-HPLC）검측방법，양품용을정제취，재용φ(갑순-이록갑완)=5∶95혼합용제경LC-NH2고상췌취주정화，이φ(을정-수)=55∶45작류동상，Shiseido C18색보주（4.6 mm×250 mm，5μm）우222 nm파장검측，외표법정량。재0.1~5.0 mg·L-1범위내，다효서봉면적여기질량농도지간정량호선성관계，상관계수위0.9995。채용전간시험방법，재번가유묘기시용불동다효서질량분수50、100、200、600 mg·kg-1，연구재불동처리시간1、6 h，1、2、3、7、14、21、30、45、60 d，다효서재번가이급토양중적잔류동태변화。첨가질량분수수평위0.05、0.1、0.5 mg·kg-1시，다효서재과실、식주화토양중적첨가회수솔분별위92.45%~103.70%、94.52%~98.85%화94.30%~102.10%，변이계수분별위3.69%~5.00%、1.58%~4.53%화1.28%~3.35%。결과표명：다효서재번가식주화토양중적강해규률균부합일급동역학방정C＝Coe-kt。당시용질량분수위600 mg·kg-1시，기재번가식주중적잔류반쇠기위1.66 d，재토양중적반쇠기위2.78 d；재식주중적강해속솔대우재토양중적강해속솔。안조추천사용적시용농도，채수시다효서재번가화토양중무잔류，증명추천시용농도시합리적。
The method for determination of the residue and degradation dynamics of paclobutrazol in tomato and soil was described. A method was established for the analysis of paclobutrazol residues in tomato and soil by using SPE-high performance liquid chromatography. The residues in the samples were extracted by acetonitrile, cleaned up by developed Supelclean LC-NH2 with the mixture of methanol-dichloromethane (5∶95,V/V) for the purification. Then, the sample were analyzed by the separation of C18 chromatographic column (4.6 mm×250 mm,5μm) with an acetonitrile and water (55∶45,V/V) mixed solution as the mobile phase and the determination with an ultraviolet detector at 222 nm. The analytes were by matrix matched standard solution, and the calibration curves shower good linearity within the concentrations of 0.1 to 5.0 mg·L-1 and the correlation coefficients were 0.9995. In field trial, The paclobutrazol of different concentrations 50, 100, 200, 600 mg·kg-1 was used on the tomato in its seeding stage,the residual dynamics were research on tomato and soil, under the different exposure of 0.04, 0.25, 1, 3, 7, 14, 21, 30, 45, 60 d. At different addition level of 0.05, 0.1, 0.5 mg·kg-1, the recovery were 92.45%~103.70%、94.52%~98.85% and 94.30%~102.10%, respectively. The CV were 3.69%~5.00%、1.58%~4.53% and 1.28%~3.35%, respectively. The results indicated that degradation of paclobutrazol in plant and soil samples were accordance with the first-order kinetic equationC=Coe-kt. When 600 mg·kg-1 of paclobutrazol was applied, the half life of tomato and soil was 1.66 d and 2.78 d. When applied according to the recommended dosage, There was no residues of paclobutrazol in the tomato can be checked in 30 d. The average growth period of tomato was 90 d. The terminal residues in tomato and siol were less than the detectable limits. So it testified that using this recommended dosage was reasonable.