浙江临床医学
浙江臨床醫學
절강림상의학
ZHEJIANG CLINICAL MEDICAL JOURNAL
2014年
7期
1030-1032
,共3页
徐加杰%葛明华%赏金标%谭卓%陈超%王可敬
徐加傑%葛明華%賞金標%譚卓%陳超%王可敬
서가걸%갈명화%상금표%담탁%진초%왕가경
鼻咽癌%转移%PIM1%mir-17-5p%细胞侵袭
鼻嚥癌%轉移%PIM1%mir-17-5p%細胞侵襲
비인암%전이%PIM1%mir-17-5p%세포침습
Nasopharyngeal carcinoma%Metastasis%PIM1%Mir-17-5p%Migration
目的：探讨PIM-1蛋白参与鼻咽癌细胞转移的机制。方法采用shRNA将CNE-2Z鼻咽癌细胞中的PIM-1的表达进行沉默，并采用realtime对mir-17-5p的表达进行检测；采用shRNA将CNE-2Z鼻咽癌细胞中的mir-17-5p的表达进行沉默，采用transwell实验比较mir-17-5p转染前后sh1-PIM-1细胞的迁移能力。结果 Realtime及Western Blot结果显示PIM-1沉默后mir-17-5p的表达水平显著升高。transwell结果显示，mir-17-5p的过表达增强CNE-2Z细胞的迁移能力。结论癌基因PIM-1通过调控mir-17-5p的表达，可以调控鼻咽癌肿瘤细胞的转移。
目的：探討PIM-1蛋白參與鼻嚥癌細胞轉移的機製。方法採用shRNA將CNE-2Z鼻嚥癌細胞中的PIM-1的錶達進行沉默，併採用realtime對mir-17-5p的錶達進行檢測；採用shRNA將CNE-2Z鼻嚥癌細胞中的mir-17-5p的錶達進行沉默，採用transwell實驗比較mir-17-5p轉染前後sh1-PIM-1細胞的遷移能力。結果 Realtime及Western Blot結果顯示PIM-1沉默後mir-17-5p的錶達水平顯著升高。transwell結果顯示，mir-17-5p的過錶達增彊CNE-2Z細胞的遷移能力。結論癌基因PIM-1通過調控mir-17-5p的錶達，可以調控鼻嚥癌腫瘤細胞的轉移。
목적：탐토PIM-1단백삼여비인암세포전이적궤제。방법채용shRNA장CNE-2Z비인암세포중적PIM-1적표체진행침묵，병채용realtime대mir-17-5p적표체진행검측；채용shRNA장CNE-2Z비인암세포중적mir-17-5p적표체진행침묵，채용transwell실험비교mir-17-5p전염전후sh1-PIM-1세포적천이능력。결과 Realtime급Western Blot결과현시PIM-1침묵후mir-17-5p적표체수평현저승고。transwell결과현시，mir-17-5p적과표체증강CNE-2Z세포적천이능력。결론암기인PIM-1통과조공mir-17-5p적표체，가이조공비인암종류세포적전이。
Objectives The purpose of this study was to investigate. Methods PIM 1 expression in CNE-2Z cell line was knocked down by shRNA,the Silence efficiency was evaluated by realtime PCR and Western Blot.The migration of nasopharyngeal carcinoma was analyzed by transwell assay. Results We found that the expression of mir-17-5p was upregulated after PIM1 knock down.Meanwhile,the migration ability was upregulated after mir-17-5p wastransfected. Conclusion PIM - 1 can promote the metastasis of nasopharyngeal carcinoma through the regulation of mir-17-5 p expression regulation.
