CAJ | 학술논문

目的：统一复方血栓通颗粒、片、滴丸和软胶囊中三七皂苷R1、人参皂苷Rg1、人参皂苷Re和人参皂苷Rb1的含量测定方法。方法采用高效液相色谱法，使用Thermo ODS2（5μm，150.0 mm×4.6 mm）色谱柱，柱温为20℃，以乙腈-水为流动相进行梯度洗脱，流速为1.0 ml/min，检测波长为203 nm。结果三七皂苷R1在0.0518～2.5900（r=0.9996）、人参皂苷Rg1在0.2090～8.3588（r=0.9991）、人参皂苷Re在0.0507～2.5371（r=0.9990）、人参皂苷Rb1在0.2044～8.1752（r=0.9995）范围内与峰面积线性关系良好；精密度、重复性、稳定性及加样回收率结果均能满足分析要求。结论该方法简单可行，为复方血栓通品种中三七皂苷R1、人参皂苷Rg1、人参皂苷Re和人参皂苷Rb1含量测定方法的统一提供了可靠的依据。
목적：통일복방혈전통과립、편、적환화연효낭중삼칠조감R1、인삼조감Rg1、인삼조감Re화인삼조감Rb1적함량측정방법。방법채용고효액상색보법，사용Thermo ODS2（5μm，150.0 mm×4.6 mm）색보주，주온위20℃，이을정-수위류동상진행제도세탈，류속위1.0 ml/min，검측파장위203 nm。결과삼칠조감R1재0.0518～2.5900（r=0.9996）、인삼조감Rg1재0.2090～8.3588（r=0.9991）、인삼조감Re재0.0507～2.5371（r=0.9990）、인삼조감Rb1재0.2044～8.1752（r=0.9995）범위내여봉면적선성관계량호；정밀도、중복성、은정성급가양회수솔결과균능만족분석요구。결론해방법간단가행，위복방혈전통품충중삼칠조감R1、인삼조감Rg1、인삼조감Re화인삼조감Rb1함량측정방법적통일제공료가고적의거。
Objective To unified the method for the content determination of Notoginsenoside R1,Ginsenoside Rg1,Ginsenoside Re,Ginsenoside Rb1 of Fufang Xueshuantong granules,tablets,dripping pils and soft capsules. Methods By HPLC method;Notoginsenoside R1,Ginsenoside Rg1,Ginsenoside Re,Ginsenoside Rb1were analyzed on an Thermo ODS2(5 μm,150.0 mm×4.6 mm)column at 20℃;The gradient elution was acetonitrile-water at the flow rate of 1.0ml/min;The detection wavelength was 203 nm.Results Notoginsenoside R1,Ginsenoside Rg1,Ginsenoside Re,Ginsenoside Rb1 al have good linearity in the range of 0.0518~2.5900(r=0.9996),0.2090~8.3588(r=0.9991), 0.0507~2.5371(r=0.9990),0.2044~8.1752(r=0.9995)with satisfied precision,repetition,stability and recovery.Conclusion This method is simple,feasible,and can offer the reliable basis for unification of the standards.