CAJ | 학술논문

目的：分析重型β地中海贫血（地贫）儿童体内自然杀伤（NK）细胞活性及受体表达，探讨输血诱导的NK细胞免疫抑制的机制。方法利用多色流式细胞技术，检测20例重型β地贫儿童、20例轻型β地贫儿童和20例健康儿童的外周血 NK 细胞 CD107a （杀伤活性）、活化型受体（NKP30、NKP46、NKG2D）及抑制型受体（NKG2A、CD158a）的表达；使用细胞内细胞因子染色检测NK细胞分泌IFN-γ的水平。结果与轻型β地贫儿童和正常儿童相比，接受长期输血的重型β地贫儿童外周血NK细胞CD107a表达明显下调（P均＜0.01），NK细胞抑制性受体NKG2A表达明显上调（P均＜0.01），NKP30、NKP46、NKG2D、CD158a及IFN-γ的表达比较差异无统计学意义（P均＞0.05）。结论 NK细胞抑制型受体NKG2A在重型β地贫输血诱导的NK细胞免疫抑制中可能发挥重要作用。
목적：분석중형β지중해빈혈（지빈）인동체내자연살상（NK）세포활성급수체표체，탐토수혈유도적NK세포면역억제적궤제。방법이용다색류식세포기술，검측20례중형β지빈인동、20례경형β지빈인동화20례건강인동적외주혈 NK 세포 CD107a （살상활성）、활화형수체（NKP30、NKP46、NKG2D）급억제형수체（NKG2A、CD158a）적표체；사용세포내세포인자염색검측NK세포분비IFN-γ적수평。결과여경형β지빈인동화정상인동상비，접수장기수혈적중형β지빈인동외주혈NK세포CD107a표체명현하조（P균＜0.01），NK세포억제성수체NKG2A표체명현상조（P균＜0.01），NKP30、NKP46、NKG2D、CD158a급IFN-γ적표체비교차이무통계학의의（P균＞0.05）。결론 NK세포억제형수체NKG2A재중형β지빈수혈유도적NK세포면역억제중가능발휘중요작용。
Objective To analyze the activity and receptor expression of peripheral natural killer (NK)cells in children with β-thalassemia major and investigate underlying mechanism of the transfusion-in-duced immunodepression. Methods Peripheral blood samples were obtained from 20 children withβ-thalasse-mia major,20 β-thalassemia minor and 20 healthy volunteers. Expression levels of CD107a,activating recep-tors (NKP30,NKP46 and NKG2D)and inhibitory receptors (NKG2A and CD158a)within circulating NK cells were detected by multicolor flow cytometry. The level of IFN-γsecreted by NK cells was measured by in-tracellular cytokine staining. Results Compared with β-thalassemia minor and healthy counterparts (all P<0.05),the cytotoxicity (CD107a expression)of circulating NK cells was remarkably down-regulated whereas NKG2A expression,as an inhibitory receptor of circulating NK cells,was significantly up-regulated in children with β-thalassemia major (all P<0.05 ). However,no statistical significance was observed in the expression of NKP30,NKP46,NKG2D,CD158a and IFN-γ(all P>0.05)among three groups. Conclusion NKG2A, as an inhibitory receptor of NK cells,may play a key role in the transfusion-induced immunodepression of NK cells in children with β-thalassemia major.