CAJ | 학술논문

背景与目的：既往研究表明，微小RNA-340(miR-340)能负性调控多种肿瘤的进展，但其在乳腺癌细胞增殖和凋亡中的研究较少，本研究旨在探讨miR-340对乳腺癌MDA-MB231细胞增殖和凋亡的作用。方法：利用脂质体LipofectamineTM2000将pre-miR-340或anti-miR-340瞬时转染至乳腺癌MDA-MB231细胞，通过RT-PCR检测miR-340 mRNA的水平，蛋白质印迹法(Western blot)检测cleaved-caspase-3蛋白的表达，MTT比色法检测细胞增殖的抑制情况，流式细胞仪检测细胞凋亡。结果：Pre-miR-340增加了MDA-MB231细胞中miR-340表达，同时增加cleaved-caspase-3蛋白表达、抑制MDA-MB231细胞增殖并促进其凋亡。而anti-miR-340抑制了MDA-MB231细胞中miR-340表达，并且抑制cleaved-caspase-3蛋白表达，促进了MDA-MB231细胞增殖，抑制了MDA-MB231细胞的凋亡。结论：miR-340转染后能上调MDA-MB231细胞中cleaved-caspase-3蛋白的表达从而抑制细胞增殖，促进其凋亡。
배경여목적：기왕연구표명，미소RNA-340(miR-340)능부성조공다충종류적진전，단기재유선암세포증식화조망중적연구교소，본연구지재탐토miR-340대유선암MDA-MB231세포증식화조망적작용。방법：이용지질체LipofectamineTM2000장pre-miR-340혹anti-miR-340순시전염지유선암MDA-MB231세포，통과RT-PCR검측miR-340 mRNA적수평，단백질인적법(Western blot)검측cleaved-caspase-3단백적표체，MTT비색법검측세포증식적억제정황，류식세포의검측세포조망。결과：Pre-miR-340증가료MDA-MB231세포중miR-340표체，동시증가cleaved-caspase-3단백표체、억제MDA-MB231세포증식병촉진기조망。이anti-miR-340억제료MDA-MB231세포중miR-340표체，병차억제cleaved-caspase-3단백표체，촉진료MDA-MB231세포증식，억제료MDA-MB231세포적조망。결론：miR-340전염후능상조MDA-MB231세포중cleaved-caspase-3단백적표체종이억제세포증식，촉진기조망。
Background and purpose:MicroRNA-340 (miR-340) has been demonstrated to play a role of negative regulation in many kinds of tumor, however, there are few reports about the relationship between miR-340 in proliferation and apoptosis of breast cancer cell. This study was aimed to explore the effect of miR-340 on proliferation and apoptosis of breast cancer cell MDA-MB231. Methods: The pre-miR-340 or anti-miR-340 were transiently transfected into breast cancer cell MDA-MB231 with LipofectamineTM2000. miR-340 level was detected by RT-PCR. The Western blot was performed to detect the protein level of cleaved-caspase-3. The inhibition rate of cell proliferation was evaluated by MTT assay. The cell apoptosis was studied by lfow cytometry. Results:The pre-miR-340 facilitated the expression of miR-340 in MDA-MB231 cells. The pre-miR-340 enhanced the protein level of cleaved-caspase-3, inhibited the proliferation of MDA-MB231 cells and increased its apoptosis. On the contrary, the expression of miR-340 was inhibited by anti-miR-340 in MDA-MB231 cells. The protein level of cleaved-caspase-3 was reduced after the anti-miR-340-transfected MDA-MB231 cells. Anti-miR-340 promoted the proliferation of MDA-MB231 cells. Decreased apoptosis of MDA-MB231 cells was observed by lfow cytometry. Conclusion:The overexpression of miR-340 can effectively inhibit the proliferation and increase the apoptosis of MDA-MB231 cells, which may be explained by up-regulating of protein cleaved-caspase-3 level.