中国癌症杂志
中國癌癥雜誌
중국암증잡지
CHINA ONCOLOGY
2014年
7期
512-516
,共5页
王天航%鲁英%杨晓燕%欧江华
王天航%魯英%楊曉燕%歐江華
왕천항%로영%양효연%구강화
乳腺癌%芳香烃受体%血清蛋白质浓度
乳腺癌%芳香烴受體%血清蛋白質濃度
유선암%방향경수체%혈청단백질농도
Breast cancer%Aryl hydrocarbon receptor%Plasma protein concentration
背景与目的：大量研究表明，活化的芳香烃受体(aryl hydrocarbon receptor，AhR)可能参与了由多环芳烃诱导的乳腺癌的发生，AhR作为公认的配体激活的转录因子，可诱导多种酶表达。本研究旨在探讨芳香烃受体和芳香烃代谢酶在人体内蛋白水平与乳腺癌及其病理指标间的关系。方法：以1∶1配对进行病例对照研究，共收集65对血样，采用酶联免疫吸附法检测血液中芳香烃受体及芳香烃代谢酶蛋白水平，同时收集病理资料。计量资料使用秩和检验进行统计分析，相关性分析使用Spearman检验。结果：秩和检验发现对照组与病例组间醛脱氢酶(aldehyde dehydrogenase，ALDH)、细胞色素(cytochrome P450，CYP450)差异无统计学意义(Z=0.196，P=0.845；Z=0.269，P=0.788)；AhR、醌氧化还原酶(quinone oxido-ereductase 1，NQO1)、谷胱甘肽-S-转移酶(glutatnione-S-transferases，GST)对照组平均浓度低于病例组，差异有统计学意义(Z=1.956，P=0.041；Z=2.627，P=0.009；Z=3.272，P=0.001)。相关性分析结果显示，病例组中AhR与GST和NQO1相关性较高(r=0.665，P<0.01；r=0.704，P<0.01)。以病理指标(ER、PR、HER-2)-、+、++、+++分组，发现以雌激素受体ER分组的AhR、GST蛋白表达水平差异有统计学意义(P<0.05)。结论：实验结果显示芳香烃受体及芳香烃代谢酶蛋白水平对乳腺癌发生可能有重要影响，检测酶蛋白水平可能对乳腺癌的早期发现有帮助，对治疗方案的选择及预后有一定意义。
揹景與目的：大量研究錶明，活化的芳香烴受體(aryl hydrocarbon receptor，AhR)可能參與瞭由多環芳烴誘導的乳腺癌的髮生，AhR作為公認的配體激活的轉錄因子，可誘導多種酶錶達。本研究旨在探討芳香烴受體和芳香烴代謝酶在人體內蛋白水平與乳腺癌及其病理指標間的關繫。方法：以1∶1配對進行病例對照研究，共收集65對血樣，採用酶聯免疫吸附法檢測血液中芳香烴受體及芳香烴代謝酶蛋白水平，同時收集病理資料。計量資料使用秩和檢驗進行統計分析，相關性分析使用Spearman檢驗。結果：秩和檢驗髮現對照組與病例組間醛脫氫酶(aldehyde dehydrogenase，ALDH)、細胞色素(cytochrome P450，CYP450)差異無統計學意義(Z=0.196，P=0.845；Z=0.269，P=0.788)；AhR、醌氧化還原酶(quinone oxido-ereductase 1，NQO1)、穀胱甘肽-S-轉移酶(glutatnione-S-transferases，GST)對照組平均濃度低于病例組，差異有統計學意義(Z=1.956，P=0.041；Z=2.627，P=0.009；Z=3.272，P=0.001)。相關性分析結果顯示，病例組中AhR與GST和NQO1相關性較高(r=0.665，P<0.01；r=0.704，P<0.01)。以病理指標(ER、PR、HER-2)-、+、++、+++分組，髮現以雌激素受體ER分組的AhR、GST蛋白錶達水平差異有統計學意義(P<0.05)。結論：實驗結果顯示芳香烴受體及芳香烴代謝酶蛋白水平對乳腺癌髮生可能有重要影響，檢測酶蛋白水平可能對乳腺癌的早期髮現有幫助，對治療方案的選擇及預後有一定意義。
배경여목적：대량연구표명，활화적방향경수체(aryl hydrocarbon receptor，AhR)가능삼여료유다배방경유도적유선암적발생，AhR작위공인적배체격활적전록인자，가유도다충매표체。본연구지재탐토방향경수체화방향경대사매재인체내단백수평여유선암급기병리지표간적관계。방법：이1∶1배대진행병례대조연구，공수집65대혈양，채용매련면역흡부법검측혈액중방향경수체급방향경대사매단백수평，동시수집병리자료。계량자료사용질화검험진행통계분석，상관성분석사용Spearman검험。결과：질화검험발현대조조여병례조간철탈경매(aldehyde dehydrogenase，ALDH)、세포색소(cytochrome P450，CYP450)차이무통계학의의(Z=0.196，P=0.845；Z=0.269，P=0.788)；AhR、곤양화환원매(quinone oxido-ereductase 1，NQO1)、곡광감태-S-전이매(glutatnione-S-transferases，GST)대조조평균농도저우병례조，차이유통계학의의(Z=1.956，P=0.041；Z=2.627，P=0.009；Z=3.272，P=0.001)。상관성분석결과현시，병례조중AhR여GST화NQO1상관성교고(r=0.665，P<0.01；r=0.704，P<0.01)。이병리지표(ER、PR、HER-2)-、+、++、+++분조，발현이자격소수체ER분조적AhR、GST단백표체수평차이유통계학의의(P<0.05)。결론：실험결과현시방향경수체급방향경대사매단백수평대유선암발생가능유중요영향，검측매단백수평가능대유선암적조기발현유방조，대치료방안적선택급예후유일정의의。
Background and purpose:Previous studies showed activated aryl hydrocarbon receptor (AhR) might be involved in the development of breast cancer induced by polycyclic aromatic hydrocarbons (PHAs) and AhR which was regarded as transcription factors activated by ligand could induced many enzymes expression. Therefore, this study aimed to explore the protein level of AhR and aryl hydrocarbon metabolic enzymes in vivo with a relationship of the breast cancer and pathology indicators. Methods:A case-control study was 1∶1 matched and 65 pairs blood samples were collected, the ELISA was used in testing blood protein levels of the aromatic hydrocarbon receptor and aryl hydrocarbon metabolic enzymes. Meanwhile pathological data was collected. The Rank sum test statistical analysis was used in analyzing measurement data, Spearman test was used in correlation analysis. Results:Statistical analysis revealed that there was no statistical difference in aldehyde dehydrogenase(ALDH), cytochrome P450(CYP450) between the case group and the control group (Z=0.196,P=0.845;Z=0.269,P=0.788);and the AhR, NQO1, GST control group concentrations were lower than that in the case group. There were statistical differences of AhR, quinone oxido-ereductase 1(NQO1), glutatnione-S-transferases(GST) between the control group and the case group(Z=1.956, P=0.041;Z=2.627, P=0.009;Z=3.272, P=0.001). Correlation analysis showed that AhR has a higher correlation in case group with GST, NQO1 (r=0.665, P<0.01;r=0.704, P<0.01). Grouping with the pathology indicators (ER, PR, HER-2)-,+,++,+++, found that the level of grouping by ER, AhR protein levels and GST protein levels have a discrepancies(P<0.05). Conclusion:These results might suggest that the protein levels of AhR and aryl hydrocarbon metabolic enzymes have a signiifcant impact on breast cancer. Detection of enzyme protein levels may be helpful for early detection of breast cancer, prompt treatment options and prognosis are meaningful.
