红外与激光工程
紅外與激光工程
홍외여격광공정
INFRARED AND LASER ENGINEERING
2014年
7期
2204-2208
,共5页
离散偶极子近似(DDA)%生物细胞%非球形粒子%光散射
離散偶極子近似(DDA)%生物細胞%非毬形粒子%光散射
리산우겁자근사(DDA)%생물세포%비구형입자%광산사
discrete dipole approximation (DDA)%biological cells%non-spherical particles%light scattering
用离散偶极子近似(Discrete Dipole Approximation，DDA)对生物细胞的光散射特性进行数值模拟，分析讨论了基于生物细胞的相对折射率、散射截面、粒子直径等的光散射图像。分析表明，生物细胞的散射光强随着散射角度和相对折射率的增大而增大，散射的主要能量集中在10°散射角以内。然后设计了用于分析悬浮生物细胞的光散射实验装置，并以悬浮的牛肾细胞作为实验样品，进行实验研究。分析结果显示，模拟数据与实验数据符合较好。为进一步研究生物细胞散射特性以及无损检测提供一种理论和实验方法。
用離散偶極子近似(Discrete Dipole Approximation，DDA)對生物細胞的光散射特性進行數值模擬，分析討論瞭基于生物細胞的相對摺射率、散射截麵、粒子直徑等的光散射圖像。分析錶明，生物細胞的散射光彊隨著散射角度和相對摺射率的增大而增大，散射的主要能量集中在10°散射角以內。然後設計瞭用于分析懸浮生物細胞的光散射實驗裝置，併以懸浮的牛腎細胞作為實驗樣品，進行實驗研究。分析結果顯示，模擬數據與實驗數據符閤較好。為進一步研究生物細胞散射特性以及無損檢測提供一種理論和實驗方法。
용리산우겁자근사(Discrete Dipole Approximation，DDA)대생물세포적광산사특성진행수치모의，분석토론료기우생물세포적상대절사솔、산사절면、입자직경등적광산사도상。분석표명，생물세포적산사광강수착산사각도화상대절사솔적증대이증대，산사적주요능량집중재10°산사각이내。연후설계료용우분석현부생물세포적광산사실험장치，병이현부적우신세포작위실험양품，진행실험연구。분석결과현시，모의수거여실험수거부합교호。위진일보연구생물세포산사특성이급무손검측제공일충이론화실험방법。
A non-destructive method was described for characterizing biological cells scattering. Firstly, the numerical simulation was performed to analyze the light scattering properties of biological cells based on the discrete dipole approximation (DDA). Then the angular dependence of the scattering intensity related to the relative refractive index, and the particle diameter were discussed in detail. The analysis shows that the scattering intensity of the living cells increases with the scattering angle and relative refractive index, and the main scattering energy is concentrated in the scattering angle less than 10° . In addition, an experimental measurement on suspended bovine kidney cells was performed and the results was compared with the simulation. Finally, the angular distribution of scattering from the simulation and experiment matched well at the angle range of 5° to 52°. This result demonstrates that DDA can be used for further theoretical research and experimental methods for biological cells scattering characteristics.
