医学临床研究
醫學臨床研究
의학림상연구
JOURNAL OF CLINICAL RESEARCH
2014年
7期
1351-1353
,共3页
李朋辉%李佳%钟玲%刘莹
李朋輝%李佳%鐘玲%劉瑩
리붕휘%리가%종령%류형
急性病%胰腺炎/代谢%膜糖蛋白类/生物合成%膜糖蛋白类/遗传学%基因表达
急性病%胰腺炎/代謝%膜糖蛋白類/生物閤成%膜糖蛋白類/遺傳學%基因錶達
급성병%이선염/대사%막당단백류/생물합성%막당단백류/유전학%기인표체
Acute Disease%Pancreatitis/metabolism%Membrane Glycoproteins/Biosynthesis%Membrane Glycoproteins/genetics%Gene Expression
【目的】探讨急性胰腺炎（AP）患者血浆可溶性髓样细胞触发受体-1（sTREM-1）水平及其基因表达与其病情严重程度的关系。【方法】收集2011年8月至2013年8月本科收治的32例轻症急性胰腺炎（MAP）和25例重症急性胰腺炎（SAP）患者及10例健康志愿者作为研究对象。采用酶联免疫吸附法（ELISA）检测患者入科后d1、d3、d7血浆sTREM-1水平，采用反转录聚合酶链式反应（RT-PCR）检测TREM-1基因表达水平，并对患者的病情进行 APACHE Ⅱ与SOFA评分。【结果】MAP组入科后d1、d3、d7血浆sTREM-1浓度分别为（386．24±190．79）ng/L、（237．22±118．64）ng/L、（143．34±92．23）ng/L ；SAP组分别为（849．56±412．31）ng/L、（674．61±347．47）ng/L、（419．56±241．57）ng/L ，两组比较差异均有统计学意义（ P ＜0．01）。血浆 sTREM-1水平与 APACHE Ⅱ评分和 SOFA评分均呈正相关，相关系数分别为（ r ＝0．619，P ＜0．05和 r ＝0．610，P ＜0．05）。【结论】sTREM-1与 AP患者病情严重程度正相关，但SAP组TREM-1mRNA基因表达未见明显增加。
【目的】探討急性胰腺炎（AP）患者血漿可溶性髓樣細胞觸髮受體-1（sTREM-1）水平及其基因錶達與其病情嚴重程度的關繫。【方法】收集2011年8月至2013年8月本科收治的32例輕癥急性胰腺炎（MAP）和25例重癥急性胰腺炎（SAP）患者及10例健康誌願者作為研究對象。採用酶聯免疫吸附法（ELISA）檢測患者入科後d1、d3、d7血漿sTREM-1水平，採用反轉錄聚閤酶鏈式反應（RT-PCR）檢測TREM-1基因錶達水平，併對患者的病情進行 APACHE Ⅱ與SOFA評分。【結果】MAP組入科後d1、d3、d7血漿sTREM-1濃度分彆為（386．24±190．79）ng/L、（237．22±118．64）ng/L、（143．34±92．23）ng/L ；SAP組分彆為（849．56±412．31）ng/L、（674．61±347．47）ng/L、（419．56±241．57）ng/L ，兩組比較差異均有統計學意義（ P ＜0．01）。血漿 sTREM-1水平與 APACHE Ⅱ評分和 SOFA評分均呈正相關，相關繫數分彆為（ r ＝0．619，P ＜0．05和 r ＝0．610，P ＜0．05）。【結論】sTREM-1與 AP患者病情嚴重程度正相關，但SAP組TREM-1mRNA基因錶達未見明顯增加。
【목적】탐토급성이선염（AP）환자혈장가용성수양세포촉발수체-1（sTREM-1）수평급기기인표체여기병정엄중정도적관계。【방법】수집2011년8월지2013년8월본과수치적32례경증급성이선염（MAP）화25례중증급성이선염（SAP）환자급10례건강지원자작위연구대상。채용매련면역흡부법（ELISA）검측환자입과후d1、d3、d7혈장sTREM-1수평，채용반전록취합매련식반응（RT-PCR）검측TREM-1기인표체수평，병대환자적병정진행 APACHE Ⅱ여SOFA평분。【결과】MAP조입과후d1、d3、d7혈장sTREM-1농도분별위（386．24±190．79）ng/L、（237．22±118．64）ng/L、（143．34±92．23）ng/L ；SAP조분별위（849．56±412．31）ng/L、（674．61±347．47）ng/L、（419．56±241．57）ng/L ，량조비교차이균유통계학의의（ P ＜0．01）。혈장 sTREM-1수평여 APACHE Ⅱ평분화 SOFA평분균정정상관，상관계수분별위（ r ＝0．619，P ＜0．05화 r ＝0．610，P ＜0．05）。【결론】sTREM-1여 AP환자병정엄중정도정상관，단SAP조TREM-1mRNA기인표체미견명현증가。
Objective To explore the relationship of plasma soluble myeloid cell triggering receptor-1 (sTREM-1) and gene expression with the severity of patients with acute pancreatitis .[Methods]A total of 32 patients with mild acute pancreatitis (MAP) ,25 patients with severe acute pancreatitis (SAP) and 10 healthy volunteers in our hospital from Aug .2011 to Aug .2013 were enrolled in the study .Enzyme-linked immu-nosorbent assay(ELISA) was used to detect plasma level of sTREM-1 at 1 ,3 and 7 days after admission .The expression of TREM-1 gene was examined by using reverse transcriptase polymerase chain reaction (RT-PCR) . APACHE Ⅱ and SOFA scores were used for assessing the condition of patients .[Results] Plasma levels of sTREM-1 in MAP group at 1 ,3 and 7 days after admission were (386 .24 ± 190 .79)ng/L ,(237 .22 ± 118 .64) ng /L and (143 .34 ± 92 .23)ng/L respectively ,and those in SAP group were (849 .56 ± 412 .31)ng/L , (674 .61 ± 347 .47)ng /L and (419 .56 ± 241 .57)ng/L respectively ,and there were significant differences be-tween two groups( P <0 .01) .Plasma levels of sTREM-1 were positively correlated with APACHE Ⅱ and SOFA score( r =0 .645 ,P <0 .05;r =0 .713 ,P <0 .05) .[Conclusion] Plasma levels of sTREM-1 is posi-tively correlated with the severity of patients with AP ,but the expression of TREM-1mRNA gene in SAP group is not increased .
