CAJ | 학술논문

【目的】探讨降钙素基因相关肽（CGRP）在内皮祖细胞（EPCs）抑制血管紧张素Ⅱ（AngⅡ）诱导的血管平滑肌细胞（VSMCs）增殖过程中的作用。【方法】采用ELISA检测EPCs表达CGRP ，对EPCs条件培养基采取CGRP抗体封闭和CGRP受体阻断后，观察EPCs条件培养基对 AngⅡ诱导的DNA合成能力、细胞总蛋白含量、细胞存活率及细胞周期的影响。【方法】EPCs能够表达并分泌高浓度的CGRP；CGRP阻断后，EPCs条件培养基对AngⅡ诱导的VSMCs DNA合成能力、细胞总蛋白含量、细胞存活率、细胞周期进程的抑制作用均较对照组明显降低（ P＜0．05）。【结论】EPCs通过旁分泌CGRP抑制AngⅡ诱导的VSMCs增殖。
【목적】탐토강개소기인상관태（CGRP）재내피조세포（EPCs）억제혈관긴장소Ⅱ（AngⅡ）유도적혈관평활기세포（VSMCs）증식과정중적작용。【방법】채용ELISA검측EPCs표체CGRP ，대EPCs조건배양기채취CGRP항체봉폐화CGRP수체조단후，관찰EPCs조건배양기대 AngⅡ유도적DNA합성능력、세포총단백함량、세포존활솔급세포주기적영향。【방법】EPCs능구표체병분비고농도적CGRP；CGRP조단후，EPCs조건배양기대AngⅡ유도적VSMCs DNA합성능력、세포총단백함량、세포존활솔、세포주기진정적억제작용균교대조조명현강저（ P＜0．05）。【결론】EPCs통과방분비CGRP억제AngⅡ유도적VSMCs증식。
Objective To explore the role of calcitonin gene-related peptide(CRGP) in endothelial progen-itor cells(EPCs) inhibiting the proliferation of vascular smooth muscle cells (VSMCs) induced by angiotensin II (AngII) .[Methods] The expression of CGRP in EPCs was determined by using ELISA .EPCs condition me-dium was blocked by CGRP antibody .The effect of EPCs condition medium on DNA synthesis ability ,total protein level ,cell survival and cell cycle induced by AngII was observed .[Results]EPCs could express and se-cret high level of CGRP .After CGRP blockage ,the inhibitory effect of EPCs condition medium on AngII-in-duced DNA synthesis ability ,total protein level ,cell survival and cell cycle progression of VSMCs was lower than that in control group( P<0 .05) .[Conclusion]EPCs inhibits the proliferation of VSMCs induced by An-gII through the paracrine secretion of CGRP .