海峡药学
海峽藥學
해협약학
STRAIT PHARMACEUTICAL JOURNAL
2014年
7期
56-57
,共2页
垂盆草片%槲皮素%山奈素%异鼠李素%HPLC
垂盆草片%槲皮素%山奈素%異鼠李素%HPLC
수분초편%곡피소%산내소%이서리소%HPLC
Cuipencao Tablets%Quercetin%Isorhamnetin%Kaempferol%HPLC
目的:建立同时测定垂盆草片中槲皮素、山奈素、异鼠李素3种成分的 HPLC 方法。方法采用 Intertsil ODS-SP色谱柱(150×4.6mm,5μm),柱温35℃,流动相为甲醇-0.4%磷酸溶液(45∶55),流速1.0mL· min -1,检测波长360nm.结果3种成分均达到基线分离,槲皮素、山奈素、异鼠李素3种成分分别在0.94μg· mL -1~37.6μg· mL -1,0.808μg· mL -1~16.16μg· mL -1,1.116μg· mL -1~22.32μg· mL -1的浓度范围内与峰面积线性关系良好,平均回收率分别为101.95%、99.01%、101.00%。结论该方法准确可靠,可用于垂盆草片的质量控制。
目的:建立同時測定垂盆草片中槲皮素、山奈素、異鼠李素3種成分的 HPLC 方法。方法採用 Intertsil ODS-SP色譜柱(150×4.6mm,5μm),柱溫35℃,流動相為甲醇-0.4%燐痠溶液(45∶55),流速1.0mL· min -1,檢測波長360nm.結果3種成分均達到基線分離,槲皮素、山奈素、異鼠李素3種成分分彆在0.94μg· mL -1~37.6μg· mL -1,0.808μg· mL -1~16.16μg· mL -1,1.116μg· mL -1~22.32μg· mL -1的濃度範圍內與峰麵積線性關繫良好,平均迴收率分彆為101.95%、99.01%、101.00%。結論該方法準確可靠,可用于垂盆草片的質量控製。
목적:건립동시측정수분초편중곡피소、산내소、이서리소3충성분적 HPLC 방법。방법채용 Intertsil ODS-SP색보주(150×4.6mm,5μm),주온35℃,류동상위갑순-0.4%린산용액(45∶55),류속1.0mL· min -1,검측파장360nm.결과3충성분균체도기선분리,곡피소、산내소、이서리소3충성분분별재0.94μg· mL -1~37.6μg· mL -1,0.808μg· mL -1~16.16μg· mL -1,1.116μg· mL -1~22.32μg· mL -1적농도범위내여봉면적선성관계량호,평균회수솔분별위101.95%、99.01%、101.00%。결론해방법준학가고,가용우수분초편적질량공제。
OBJECTIVE To establish a HPLC method for simultaneous determination of three chemical compo-nents ( quercetin ,isorhamnetin ,kaempferol ) in Chuipencao Tablets.METHODS The HPLC was performed on an Intertsil ODS-SP column (150 ×4.6mm,5μm)at 35℃ with methol-0.4%phosphoric acid solution (45∶55)as the mobile phase ,and detected at 360 nm with flow rate of 1.0 mL· min -1.RESULTS Three compounds were separa-ted perfectly ,linearities of quercetin ,isorhamnetin ,kaempferol were in ranges of 0.94μg · mL-1 ~37.6μg · mL-1 , 0.808μg· mL-1 ~16.16μg· mL-1 ,1.116μg· mL-1 ~22.32μg· mL-1 ,respectively.The average recoveries were 101.95%、99.01%、101.00%, respectively.CONCLUSION The method is accutae and reliable for the quality control of Cuipencao Tablets.