CAJ | 학술논문

目的：研究重复测试和不同性别两个因素对小鼠在旷场试验中自主行为活动和焦虑行为的影响。方法通过动物视频行为分析系统，分别记录和分析雌雄小鼠5 min内在旷场中的总路程、总体平均速度、总体平行指数、中央区的路程占总路程的比例、中央区的平均速度、中央区进入次数、中央区停留时间占整体测试时间的比例和中央区平行指数，每天测试1次，共测试3d。结果在旷场试验中，小鼠的总路程、总的平均速度、总的平行指数、中央区路程占总路程的比例、中央区的平均速度、进入中央区次数、中央区停留时间和中央区的平行指数，随测试次数的增加呈下降趋势（均为P＜0.001）；整体而言，以上指标在雌雄小鼠之间无显著性差异（均为P＞0.05），但随测试次数增加，雄性小鼠的下降趋势似乎比雌性小鼠明显，尤其在第三次测试中，雄性小鼠在中央区的平均速度（P＜0.05）和平行指数（P＜0.01）比雌性小鼠更低。结论重复测试旷场试验会影响小鼠的自主行为活动，同时也会干扰焦虑状态的评价；不同性别对小鼠的初次旷场试验测试无影响，但重复测试对雄性小鼠的自主行为活动的影响似乎比雌性小鼠更大。
목적：연구중복측시화불동성별량개인소대소서재광장시험중자주행위활동화초필행위적영향。방법통과동물시빈행위분석계통，분별기록화분석자웅소서5 min내재광장중적총로정、총체평균속도、총체평행지수、중앙구적로정점총로정적비례、중앙구적평균속도、중앙구진입차수、중앙구정류시간점정체측시시간적비례화중앙구평행지수，매천측시1차，공측시3d。결과재광장시험중，소서적총로정、총적평균속도、총적평행지수、중앙구로정점총로정적비례、중앙구적평균속도、진입중앙구차수、중앙구정류시간화중앙구적평행지수，수측시차수적증가정하강추세（균위P＜0.001）；정체이언，이상지표재자웅소서지간무현저성차이（균위P＞0.05），단수측시차수증가，웅성소서적하강추세사호비자성소서명현，우기재제삼차측시중，웅성소서재중앙구적평균속도（P＜0.05）화평행지수（P＜0.01）비자성소서경저。결론중복측시광장시험회영향소서적자주행위활동，동시야회간우초필상태적평개；불동성별대소서적초차광장시험측시무영향，단중복측시대웅성소서적자주행위활동적영향사호비자성소서경대。
OBJECTIVE To study the effect on the locomotory activity and anxiety-like behavior of laboratory mice by both factors of repeated measurement and sex.METHODS The total distance traveled ,total mean velocity , total parallel index,the percentage of distance in center zone ,the mean velocity in center zone ,the center zone en-tries,the percentage of time spent in center zone and the parallel index in center zone of the female and male mice for 5 minutes were recorded and analyzed in open field test by animal video tracking analysis system ,once a day for 3 days.RESULTS In the open field test ,the indexes of the total distance traveled ,total mean velocity ,total parallel index ,the percentage of distance in center zone ,the mean velocity in center zone ,the center zone entries ,the percent-age of time spent in center zone and the parallel index in center zone in mice showed the downtrend as the measure -ment numbers were increasing ( P<0.001 ).In general,the sex did not make the indexes mentioned above signifi-cantly different ( P>0.05 ).However ,as repeating the tests ,the downtrend of these in male mice were seemly more significant than female mice ,especially in the third time measurement ,in which the mean velocity ( P<0.05 ) and parallel index ( P<0.01 ) in center zone were lower than the ones in female mice ,respectively.CONCLUSION The repeated measurement inferences the locomotory activity and interrupted the anxiety state of mice.Sex of mice does not make the behavior in open field different at the first time ,but the repeated measurement seemly more signifi-cantly affect the locomotory activity of the male mice than the female.