CAJ | 학술논문

为贵州黑山羊垂体特异性转录因子（Pituitary Specific Transcriptiona Factorl，POU1/Pit-1）与胴体性状相关的分子标记辅助选择提供参考，运用 PCR及测序技术对贵州黑山羊POU1F1基因第4内含子进行了多态性检测。结果表明：贵州黑山羊第4内含子存在2个突变位点，即第222位点发生 C→T的突变（C222T），产生CC和Cc 2种基因型；第685位点发生G→A的突变（G685A），产生DD和Dd 2种基因型。CC、DD基因型频率均为0．967，C、c基因频率分别为0．983和0．017，D、d基因频率分别为0．983和0．017。等位基因C、D是相应突变位点的优势等位基因，CC、DD基因型是相应突变位点的优势基因型。贵州黑山羊POU1F1基因第4内含子的突变位点达到 Hardy-Weinberg平衡。
위귀주흑산양수체특이성전록인자（Pituitary Specific Transcriptiona Factorl，POU1/Pit-1）여동체성상상관적분자표기보조선택제공삼고，운용 PCR급측서기술대귀주흑산양POU1F1기인제4내함자진행료다태성검측。결과표명：귀주흑산양제4내함자존재2개돌변위점，즉제222위점발생 C→T적돌변（C222T），산생CC화Cc 2충기인형；제685위점발생G→A적돌변（G685A），산생DD화Dd 2충기인형。CC、DD기인형빈솔균위0．967，C、c기인빈솔분별위0．983화0．017，D、d기인빈솔분별위0．983화0．017。등위기인C、D시상응돌변위점적우세등위기인，CC、DD기인형시상응돌변위점적우세기인형。귀주흑산양POU1F1기인제4내함자적돌변위점체도 Hardy-Weinberg평형。
The polymorphism of the fourth intron of POU1F1 gene in Guizhou black goat was detected by PCR and sequencing technique to provide a reference for marker-assisted selection of POU1F1 gene related to carcass trait in Guizhou black goat.There are two mutation loci in the fourth intron of Guizhou black goat,C on 222th nucleotide is mutated to T,which produces CC and Cc genotypes.G on 685 bp is mutated to A,which produces DD and Dd genotypes.The frequency of CC,DD,C,c,D and d genes is 0.967,0.967,0.983,0.017,0.983 and 0.017 respectively.Allelic gene C and D is the dominant allelic gene at the relevant mutation locus separately.CC and DD genotype is the dominant genotype at the relevant mutation locus respectively also.The mutation loci of the fourth intron of POU1F1 gene in Guizhou black goat reaches Hardy-Weinberg equilibrium.