CAJ | 학술논문

为探明鸭LXRα基因的功能并为该基因在鸭育种中的运用提供参考，采用实时荧光定量PCR法检测樱桃谷鸭LXRαmRNA的组织表达谱，分析LXRαmRNA表达水平与樱桃谷鸭肉质和血清生化指标的相关性。结果表明：胸肌pH、失水率、嫩度、肌内脂肪和水分等肉质指标在公、母鸭间均未达到显著差异（P＞0．05），血清中的总蛋白、球蛋白、甘油三酯和总胆固醇公鸭显著高于母鸭（P＜0．05）；LXRαmRNA在肝脏等11个组织中均存在不同程度的表达，在肝脏中表达量最高；LXRαmRNA表达量与甘油三酯和总胆固醇含量呈极显著正相关（P＜0．01），与其他血清指标和肉质指标的相关性不显著（P＞0．05）。鸭LXRα基因可作为脂质性状选择的候选基因。
위탐명압LXRα기인적공능병위해기인재압육충중적운용제공삼고，채용실시형광정량PCR법검측앵도곡압LXRαmRNA적조직표체보，분석LXRαmRNA표체수평여앵도곡압육질화혈청생화지표적상관성。결과표명：흉기pH、실수솔、눈도、기내지방화수분등육질지표재공、모압간균미체도현저차이（P＞0．05），혈청중적총단백、구단백、감유삼지화총담고순공압현저고우모압（P＜0．05）；LXRαmRNA재간장등11개조직중균존재불동정도적표체，재간장중표체량최고；LXRαmRNA표체량여감유삼지화총담고순함량정겁현저정상관（P＜0．01），여기타혈청지표화육질지표적상관성불현저（P＞0．05）。압LXRα기인가작위지질성상선택적후선기인。
To investigate the duck LXRαgene function and provide a reference for duck molecular breeding,tissue expression profile of Cherry Valley duck LXRαgene mRNA were detected by relative quantification PCR (RQ-PCR),and correlation analysis were performed between LXRαmRNA expression and meat quality and serum biochemical indexes. The results showed that the meat quality traits of different gender had no significant difference (P>0.05).In serum total protein,globulin,triglyceride and total cholesterol of different gender had significant difference(P<0.05).LXRαmRNA were expressed in eleven tissues,and was the highest in the liver.The expression levels of LXRαmRNA had significant positive correlation with serum triglyceride and total cholesterol(P<0.01),and no significant correlation with all meat quality traits (P>0.05 ).The study indicated that LXRαgene could be treated as the candidate gene of lipid traits in ducks.