CAJ | 학술논문

为红托竹荪优良菌种的选育和开发利用提供参考，利用ITS和ISSR分子标记对18个贵州红托竹荪种质的遗传多样性进行分析。结果表明：1）18个红托竹荪菌株的ITS序列长度为611～621 bp，GC碱基的含量为53．9％～54．68％，相似性为99．9％～100％。2）在遗传相似度为0．77时，将18个红托竹荪菌株分为2个类群。第Ⅰ类群包含13个菌株，分别为3个栽培菌株（ZS017，ZS036，ZS038）和10个野生菌株（YZS010-1，YZS010-2，YZS012，YZS018，YZS033，YZS035，YZS039，YZS041，YZS044，YZS046）；第Ⅱ类群包含5个野生菌株（YZS040，YZS043，YZS045，YZS047，YZS052）。结论，贵州18个红托竹荪种质的亲缘关系较近，没有明显的地域分布特点，其遗传背景差异较小。
위홍탁죽손우량균충적선육화개발이용제공삼고，이용ITS화ISSR분자표기대18개귀주홍탁죽손충질적유전다양성진행분석。결과표명：1）18개홍탁죽손균주적ITS서렬장도위611～621 bp，GC감기적함량위53．9％～54．68％，상사성위99．9％～100％。2）재유전상사도위0．77시，장18개홍탁죽손균주분위2개류군。제Ⅰ류군포함13개균주，분별위3개재배균주（ZS017，ZS036，ZS038）화10개야생균주（YZS010-1，YZS010-2，YZS012，YZS018，YZS033，YZS035，YZS039，YZS041，YZS044，YZS046）；제Ⅱ류군포함5개야생균주（YZS040，YZS043，YZS045，YZS047，YZS052）。결론，귀주18개홍탁죽손충질적친연관계교근，몰유명현적지역분포특점，기유전배경차이교소。
To provide a reference for breeding and utilizing D.rubrovolvata,the authors analyzed the genetic diversity of D.rubrovolvata strains isolated from Guizhou by ITS and ISSR.Results:1 )ITS sequence length of 18 D.rubrovolvata strains was 611~621bp,GC base content was 53.9%~53.9% and the similarity was 99.9%~100%.2)The clustering analysis of ISSR showed that these strains could be divided into two groups at a similarity level of 0.77.GroupⅠcontained 13 strains,of which there were three cultivated strains(ZS017,ZS036,ZS038)and 10 wild strains(YZS010-1,YZS010-2,YZS012, YZS018,YZS033,YZS035,YZS039,YZS041,YZS044,YZS046).GroupⅡ contained five wild strains (YZS040,YZS043,YZS045,YZS047,YZS052 ).In conclusion,the genetic relationship of these 18 strains was close,and there was not obvious regional distribution characteristics.The genetic background was of little difference.