CAJ | 학술논문

目的：建立高效液相色谱测定火麻仁药材中胡芦巴碱含量的方法，为2015版药典火麻仁指标性成分的含量测定项提供依据。方法：采用Hypersil氨基柱，流动相乙腈－水（80∶20，V∶V），检测波长265nm。结果：胡芦巴碱进样量在0.104~1.04μg范围内线性关系良好，r=0.9999（n=6），加样回收率100.7%，RSD=1.56%（n=9）；10批火麻仁药材中胡芦巴碱的含量为0.6504～0.9931mg·g-1。结论：胡芦巴碱为火麻仁药材及饮片中的特征成分，其提取及含测方法操作简便，结果可靠，可作为火麻仁药材质量控制的指标性成分。
목적：건립고효액상색보측정화마인약재중호호파감함량적방법，위2015판약전화마인지표성성분적함량측정항제공의거。방법：채용Hypersil안기주，류동상을정－수（80∶20，V∶V），검측파장265nm。결과：호호파감진양량재0.104~1.04μg범위내선성관계량호，r=0.9999（n=6），가양회수솔100.7%，RSD=1.56%（n=9）；10비화마인약재중호호파감적함량위0.6504～0.9931mg·g-1。결론：호호파감위화마인약재급음편중적특정성분，기제취급함측방법조작간편，결과가고，가작위화마인약재질량공제적지표성성분。
Objective: An HPLC method for determination of trigonelline in fruit of Cannabis sativa was devel-oped, to provide a quality control method for 2015 edition of Chinese Pharmacopoeia. Methods: The chromatogra-phy was carried out on a Hypersil amino column(250mm×4.6mm, 5μm)with acetonitrile-water(80:20, V:V)as the mobile phase, the detection wavelength was set at 265nm. Results: Trigonelline could be successfully deter-mined and the linear rang was 0.104~1.04μg (r=0.9999). The average recoveries (n = 9)of the trigonelline was 100.7%,RSD=1.56%. The content of trigonelline in 10 batches was 0.6504~0.9931mg ·g -1. Conclusion:Trigonelline was indeed the specific ingredient in fruit and seed of Cannabis sativa. The method is accurate, reli-able and sensitive for quality control of fruit of cannabis sativa.