CAJ | 학술논문

利用葡萄糖酸钠在强酸性条件下形成内酯，通过羟胺-三氯化铁显色法生成红棕色异羟肟酸-Fe3+络合物，进行分光光度检测。建立标准曲线，其线性方程为y=113.0833x+0.1162，R2=0.9999，检测波长为500nm，在1×10-3~9×10-3mol·L-1范围内具有良好的线性关系，平均加标回收率为99.79%，并且该方法能够检测Au/γ-Al2O3催化葡萄糖氧化反应液中葡萄糖酸钠的含量。
이용포도당산납재강산성조건하형성내지，통과간알-삼록화철현색법생성홍종색이간우산-Fe3+락합물，진행분광광도검측。건립표준곡선，기선성방정위y=113.0833x+0.1162，R2=0.9999，검측파장위500nm，재1×10-3~9×10-3mol·L-1범위내구유량호적선성관계，평균가표회수솔위99.79%，병차해방법능구검측Au/γ-Al2O3최화포도당양화반응액중포도당산납적함량。
Based on the ability of sodium gluconate can form the corresponding lactone at pH 1.5, then gluco-lactone can react with hydroxylamine-ferric trichloride to form red-brown hydroxamate Fe 3+complex, so the con-tent of complex can be determined by colorimetry and the standard curve is established. The corresponding linear equation is y=113.0833x+0.1162, R2=0.9999. The maximum absorbance of the complex is at 500nm.There is a well linearity in the range of 1×10-3~9×10-3mol·L-1 and the average recovery is 99.79%. This method can be successful-ly applied to the detection of sodium gluconate which is formed by the oxidation of glucose over Au/γ-Al2O3.