CAJ | 학술논문

目的：建立维药睡莲花中没食子酸和烟花苷含量的高效液相色谱测定方法。方法色谱柱：Wondasil C18(250 mm×4.6 mm,5μm)；流动相：A为甲醇,B为0.1%冰乙酸混合水溶液,梯度洗脱；流速：1.0 mL/min；检测波长：270 nm；柱温：30℃。结果没食子酸及烟花苷进样量分别在0.065～0.585μg(r＝0.9991)、0.133～1.197μg(r＝0.9995)范围内与峰面积呈良好的线性关系,平均回收率分别为102.71%、102.08%,RSD分别为1.97%、0.46%。结论本方法操作简单、准确可靠,可用于维药睡莲花中烟花苷和没食子酸含量的测定。
목적：건립유약수연화중몰식자산화연화감함량적고효액상색보측정방법。방법색보주：Wondasil C18(250 mm×4.6 mm,5μm)；류동상：A위갑순,B위0.1%빙을산혼합수용액,제도세탈；류속：1.0 mL/min；검측파장：270 nm；주온：30℃。결과몰식자산급연화감진양량분별재0.065～0.585μg(r＝0.9991)、0.133～1.197μg(r＝0.9995)범위내여봉면적정량호적선성관계,평균회수솔분별위102.71%、102.08%,RSD분별위1.97%、0.46%。결론본방법조작간단、준학가고,가용우유약수연화중연화감화몰식자산함량적측정。
Objective To establish an HPLC method for the content determination of gallic acid and kaempferol-3-O-rutinoside inNymphaea candida Presl..MethodsThe separation was carried out on a Wondasil C18 column (250 mm×4.6 mm, 5μm). The mobile phase was methanol-0.1% acetic acid solution, with gradient elution;the flow rate was 1.0 mL/min;the detection wavelength was set at 270 nm;the column temperature was 30℃.Results The linear ranges of gallic acid and kaempferol-3-O-rutinoside were 0.065-0.585μg (r=0.999 1), 0.133-1.197μg (r=0.999 5), respectively. The average recoveries of gallic acid and kaempferol-3-O-rutinoside were 102.71%, 102.08%, with RSD of 1.97%, 0.46%, respectively.Conclusion This method was rapid, accurate, and reliable. It can be used for the determination of allic acid and kaempferol-3-O-rutinoside in Nymphaea candidaPresl..