CAJ | 학술논문

目的：建立头花蓼GAP基地产头花蓼药材的高效液相色谱(HPLC)特征图谱,为从整体上控制种植头花蓼的品质提供参考依据。方法采用DiamonsiL C18色谱柱(250 mm×4.6 mm,5μm),以甲醇-0.2%磷酸水为流动相梯度洗脱,流速为1.0 mL/min,柱温25℃,检测波长为254 nm。结果建立了头花蓼GAP基地产头花蓼药材HPLC特征图谱,10批样品的相似度均在90%以上,并确定了11个共有峰。结论所建立的方法可用于头花蓼GAP基地产药材特征图谱测定,为从整体上控制头花蓼药材品质及质量标准的制定提供了依据。
목적：건립두화료GAP기지산두화료약재적고효액상색보(HPLC)특정도보,위종정체상공제충식두화료적품질제공삼고의거。방법채용DiamonsiL C18색보주(250 mm×4.6 mm,5μm),이갑순-0.2%린산수위류동상제도세탈,류속위1.0 mL/min,주온25℃,검측파장위254 nm。결과건립료두화료GAP기지산두화료약재HPLC특정도보,10비양품적상사도균재90%이상,병학정료11개공유봉。결론소건립적방법가용우두화료GAP기지산약재특정도보측정,위종정체상공제두화료약재품질급질량표준적제정제공료의거。
Objective To establish HPLC characteristic spectrum of Polygonum Capitatum from GAP planting base, and provide reference for the overall quality control of Polygonum Capitatum. Methods HPLC analysis was performed on a DiamonsiL C18 chromatographic column (250 mm× 4.6 mm, 5μm) with the eluting system of gradient consisted of methanol and 0.2% H3PO4. The flow rate was 1.0 mL/min. The column temperature was maintained at 25℃ and the detection wavelength was at 254 nm.Results HPLC characteristic spectrum of Polygonum Capitatum samples from 10 different areas was established, which contained 11 common peaks, and the similarities of comparative results were over 90%.Conclusion The established method can be used to determine the characteristic spectrum of Polygonum Capitatum and can provide a basis for the overall quality control and quality standards of Polygonum Capitatum.