中国中医药信息杂志
中國中醫藥信息雜誌
중국중의약신식잡지
CHINESE JOURNAL OF INFORMATION ON TRADITIONAL CHINESE MEDICINE
2014年
8期
60-62,63
,共4页
肥胖%瘦素%瘦素抵抗%神经肽Y%细胞因子转录负调节因子%升清药物%健脾理气药物%大鼠
肥胖%瘦素%瘦素牴抗%神經肽Y%細胞因子轉錄負調節因子%升清藥物%健脾理氣藥物%大鼠
비반%수소%수소저항%신경태Y%세포인자전록부조절인자%승청약물%건비리기약물%대서
obesity%leptin%leptin resistance%eunropeptide Y%suppressors of cytokine signaling-3%Shengqing herbs%Jianpi Liqi herbs%rats
目的：探讨升清中药抑制饮食诱导肥胖(DIO)大鼠肥胖及瘦素抵抗的作用机制。方法取 Wistar大鼠,制作DIO大鼠40只和DIO抵抗(DIO-R)大鼠10只。DIO大鼠分为模型组、西布曲明组、健脾理气组、升清组,每日分别以生理盐水、西布曲明、健脾理气药物(陈皮、木香、枳实)、升清药物(柴胡、升麻、葛根)灌胃,空白组与DIO-R组予生理盐水,灌胃16周。测定干预前后体质量,检测干预后血清神经肽Y(NPY)、瘦素和脂肪瘦素、细胞因子信号传导抑制因子3(SOCS-3)。结果与DIO-R组比较,模型组体质量增加、NPY升高(P＜0.01),血清和脂肪瘦素降低(P＜0.05),SOCS-3升高(P＜0.05)。药物干预后,各药物组体质量有所下降。健脾理气组脂肪瘦素升高(P＜0.05)；升清组在升高瘦素、降低体质量和 NPY、SOCS-3水平方面,优于西布曲明组和健脾理气组,差异有统计学意义(P＜0.05,P＜0.01)。结论升清药物能减轻肥胖,抑制瘦素抵抗。
目的：探討升清中藥抑製飲食誘導肥胖(DIO)大鼠肥胖及瘦素牴抗的作用機製。方法取 Wistar大鼠,製作DIO大鼠40隻和DIO牴抗(DIO-R)大鼠10隻。DIO大鼠分為模型組、西佈麯明組、健脾理氣組、升清組,每日分彆以生理鹽水、西佈麯明、健脾理氣藥物(陳皮、木香、枳實)、升清藥物(柴鬍、升痳、葛根)灌胃,空白組與DIO-R組予生理鹽水,灌胃16週。測定榦預前後體質量,檢測榦預後血清神經肽Y(NPY)、瘦素和脂肪瘦素、細胞因子信號傳導抑製因子3(SOCS-3)。結果與DIO-R組比較,模型組體質量增加、NPY升高(P＜0.01),血清和脂肪瘦素降低(P＜0.05),SOCS-3升高(P＜0.05)。藥物榦預後,各藥物組體質量有所下降。健脾理氣組脂肪瘦素升高(P＜0.05)；升清組在升高瘦素、降低體質量和 NPY、SOCS-3水平方麵,優于西佈麯明組和健脾理氣組,差異有統計學意義(P＜0.05,P＜0.01)。結論升清藥物能減輕肥胖,抑製瘦素牴抗。
목적：탐토승청중약억제음식유도비반(DIO)대서비반급수소저항적작용궤제。방법취 Wistar대서,제작DIO대서40지화DIO저항(DIO-R)대서10지。DIO대서분위모형조、서포곡명조、건비리기조、승청조,매일분별이생리염수、서포곡명、건비리기약물(진피、목향、지실)、승청약물(시호、승마、갈근)관위,공백조여DIO-R조여생리염수,관위16주。측정간예전후체질량,검측간예후혈청신경태Y(NPY)、수소화지방수소、세포인자신호전도억제인자3(SOCS-3)。결과여DIO-R조비교,모형조체질량증가、NPY승고(P＜0.01),혈청화지방수소강저(P＜0.05),SOCS-3승고(P＜0.05)。약물간예후,각약물조체질량유소하강。건비리기조지방수소승고(P＜0.05)；승청조재승고수소、강저체질량화 NPY、SOCS-3수평방면,우우서포곡명조화건비리기조,차이유통계학의의(P＜0.05,P＜0.01)。결론승청약물능감경비반,억제수소저항。
Objective To observe the effects of Shengqing Chinese herbs on the obesity and leptin resistance of diet-induced obesity (DIO) rats.Methods Wistar rats were produced a rat model including of 40 DIO rats and 10 DIO-R rats. The DIO rats were divided into DIO model group, sibutramine group, Jianpi Liqi group and Shengqing group, which were respectively by gavage of saline, sibutramine, Jianpi Liqi herbs (Citri reticulatae pericarpium, Aucklandiae radix, Aurantii fructus immaturus), Shengqing herbs (Bupleuri radix, Cimicifugae rhizoma, Puerariae lobatae radix), blank control group and DIO-R group were by gavage of saline. Their weight, were examined after 16 weeks gavage. Leptin and neuropeptide Y in the serum were examined. Leptin, TNF-α, and suppressors of cytokine signaling-3 (SOCS-3) in the homogenized fat were examined. Results Compared with the DIO-R group, DIO model group's body weight, NPY significantly increased (P<0.01);leptin in the serum and homogenized fat decreased (P<0.05);SOCS-3 increased (P<0.05). After drug intervention, weight of each drug group declined. Compared with the DIO model group, Jianpi Liqi group's leptin in the homogenized fat increased (P<0.05). Shengqing group's leptin in homogenized fat increased, body weight reduced, NPY and SOCS-3 levels decreased, and better than sibutramine group and Jianpi Liqi group (P<0.05,P<0.01).Conclusion Shengqing herbs can reduce obesity and inhibit leptin resistance.
