CAJ | 학술논문

目的：观察三七总皂苷对β-淀粉样蛋白(Aβ)42损伤脑微血管内皮细胞ZO-1表达的影响,为阿尔茨海默病的对因治疗提供新的依据。方法细胞分为正常对照组、模型组和三七总皂苷低、中、高剂量组,37℃、5%CO2培养箱中培养24 h后,采用MTT比色法检测细胞活力,Western blot检测ZO-1蛋白表达。结果 Aβ42刺激后微血管内皮细胞活力降低(P＜0.01),ZO-1蛋白表达被抑制(P＜0.01)；与模型组比较,三七总皂苷低、中、高剂量组微血管内皮细胞活力增加,其中三七总皂苷中、高剂量组增加较为明显,差异有统计学意义(P＜0.05),ZO-1蛋白表达增加。结论三七总皂苷通过抑制Aβ42所致微血管内皮细胞活力降低,恢复血脑屏障的部分屏障功能。
목적：관찰삼칠총조감대β-정분양단백(Aβ)42손상뇌미혈관내피세포ZO-1표체적영향,위아이자해묵병적대인치료제공신적의거。방법세포분위정상대조조、모형조화삼칠총조감저、중、고제량조,37℃、5%CO2배양상중배양24 h후,채용MTT비색법검측세포활력,Western blot검측ZO-1단백표체。결과 Aβ42자격후미혈관내피세포활력강저(P＜0.01),ZO-1단백표체피억제(P＜0.01)；여모형조비교,삼칠총조감저、중、고제량조미혈관내피세포활력증가,기중삼칠총조감중、고제량조증가교위명현,차이유통계학의의(P＜0.05),ZO-1단백표체증가。결론삼칠총조감통과억제Aβ42소치미혈관내피세포활력강저,회복혈뇌병장적부분병장공능。
Objective To study the effects of Panax Notoginseng Saponins (PNS) on the damaged expression of ZO-1 of brain microvascular endothelial cells caused by Aβ42.MethodsBrain microvascular endothelial cells were divided into normal control group, model group, PNS low-, medium- and high concentration groups. They were incubated for 24h in 5% CO2 incubator at 37℃ . Then cell vitality was detected by MTT colorimetric method and ZO-1 protein expression was tested by Western blot.Results Stimulation of Aβ42 reduced the activity of microvascular endothelial cells (P<0.01) and suppressed the expression of ZO-1 protein (P<0.01). Compared with the model group, the activity of microvascular endothelial cells of PNS groups, especially the high and medium dose groups (P<0.05), and increased the ZO-1 protein expression. Conclusion PNS can partly recover the barrier function of blood brain barrier through inhibiting the decrease of the activity of microvascular endothelial cells caused by Aβ42.